低温胁迫对草菇菌株VG39生理及转录组影响  

Effects of Low-Temperature Stress on Physiology and Transcriptome of Volvariella volvacea VG39

作　　者：张童言 郑婷婷 万佳宁[2] 李正鹏[2] 龚明 张建国[1] ZHANG Tongyan;ZHENG Tingting;WAN Jianing;LI Zhengpeng;GONG Ming;ZHANG Jianguo(Institute of Food Science and Engineering,School of Health Science and Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China;Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Key Laboratory of Edible Fungi Resources and Utilization(South),Ministry of Agriculture and Rural Affairs,P.R.China,National Engineering Research Center of Edible Fungi,National R&D Center for Edible Fungi Processing,Key Laboratory of Agricultural Genetics and Breeding of Shanghai,Shanghai 201403,China;School of Food Science,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]上海理工大学健康科学与工程学院食品科学与工程研究所,上海200093 [2]上海市农业科学院食用菌研究所,农业农村部南方食用菌资源利用重点实验室,国家食用菌工程技术研究中心,国家食用菌加工技术研发分中心,上海市农业遗传育种重点实验室,上海201403 [3]上海海洋大学食品学院,上海201306

出　　处：《食用菌学报》2025年第1期10-17,共8页Acta Edulis Fungi

基　　金：国家自然科学基金(32072642)。

摘　　要：对草菇(Volvariella volvacea)菌株VG39和V23(对照)的菌丝进行低温(4℃)处理,检测处理前后多糖、赖氨酸、可溶性蛋白、总游离氨基酸含量,并进行红外检测、转录组分析。结果表明:未经低温处理时,与菌株V23比较,菌株VG39多糖和赖氨酸含量较高;与未经低温处理比较,两个菌株多糖、赖氨酸、可溶性蛋白、总游离氨基酸含量均显著降低。与未经低温处理比较,菌株VG39红外光谱中的吸收峰明显减少;有882个上调差异表达基因(DEGs),核糖体中的DEGs数量(2个上调表达基因和12个下调表达基因)最多,甘氨酸、丝氨酸、苏氨酸代谢通路的DEGs数量(8个上调表达基因)较多。研究结果为分析菌株VG39对低温耐受机制提供参考。Volvariella volvacea VG39 is a mutant strain previously obtained through mutagenesis of V.volvacea V23 by atmospheric pressure room temperature plasma.It exhibits a certain level of tolerance to low-temperature stress,but the molecular characteristics in response to low-temperature stress remain unclear.Both VG39 and V23(control)were incubated under low temperature(4℃)and then measured for contents of polysaccharides,lysine,soluble protein and total free amino acids in the mycelia,detected for infrared spectrum,and analyzed for transcriptome.The results showed that VG39 contained higher levels of polysaccharides and lysine than V23 before 4℃ treatment.After 4℃ treatment for 8 h,both strains contained significantly lower levels of polysaccharides,lysine,soluble protein,and total amino acids compared with those without 4℃ treatment.The number of the infrared absorption peaks in VG39 was also significantly decreased after 4℃ treatment for 8 h.Compared with before 4℃ treatment,there were 882 differentially expressed genes(DEGs)up-regulated in VG39 after 4℃ treatment for 8 h.The number of DEGs in ribosomes(2 up-regulated genes and 12 down-regulated genes)was the greatest,followed by the number of DEGs in the glycine,serine and threonine metabolic pathway(8 up-regulated genes).These results provided a reference for understanding the underlying mechanism of VG39 in response to low-temperature stress.

关 键 词：草菇 低温胁迫 多糖 赖氨酸 红外光谱 转录组 

分 类 号：S646.13[农业科学—蔬菜学]