miR-508-3p通过调控ZEB1参与卵巢癌进展的研究  

Research of miR-508-3p involvement in ovarian cancer progression by regulating ZEB1

作　　者：徐玉红 朱帅莹 单江静[1] 郑伟平[1] 张慧雅[1] 王运根[1] XU Yu-hong;ZHU Shuai-ying;SHAN Jiang-jing;ZHENG Wei-ping;ZHANG Hui-ya;WANG Yun-gen(Department of Gynecology,Shaoxing People's Hospital,Shaoxing 312000,Zhejiang Province,China)

机构地区：[1]绍兴市人民医院妇科,浙江绍兴312000

出　　处：《中国临床药理学杂志》2025年第2期193-197,共5页The Chinese Journal of Clinical Pharmacology

基　　金：浙江省医药卫生科技计划基金资助项目(2023KY12502023KY362)。

摘　　要：目的研究微小RNA-508-3p(miR-508-3p)在上皮性卵巢癌(EOC)癌组织中的表达情况,对卵巢癌细胞迁移、侵袭能力的影响,及其与锌指E盒结合蛋白1(ZEB1)的调控关系。方法收集手术切除的EOC癌组织及配对的癌旁正常组织。将SKOV3细胞分为NC mimic组(转染NC mimic)、miR-508-3p mimic组(转染miR-508-3p mimic)、si-NC组(转染si-NC)、si-ZEB1组(转染si-ZEB1)和共转染组(共转染si-ZEB1和miR-508-3p mimic)。用实时荧光定量聚合酶链反应法检测EOC癌组织及癌旁正常组织与5组细胞中miR-508-3p和ZEB1 mRNA的表达水平,用Transwell实验法检测细胞的迁移、侵袭能力。结果EOC癌组织和癌旁正常组织中miR-508-3p相对表达水平分别为0.77±0.36和1.07±0.40,ZEB1 mRNA相对表达水平分别为2.10±1.21和1.29±0.95,在统计学上差异均有统计学意义(均P<0.01)。NC mimic组、miR-508-3p mimic组、si-NC组、si-ZEB1组和共转染组的迁移细胞数分别为(633.00±32.49)、(319.20±19.89)、(650.40±25.85)、(375.00±17.25)和(129.40±17.10)个,侵袭细胞数分别为(527.20±25.01)、(288.60±16.68)、(520.00±25.83)、(293.40±18.37)和(76.60±8.76)个,miR-508-3p相对表达水平分别为1.05±0.37、3.94±1.21、1.01±0.21、1.26±0.34和3.40±0.41,ZEB1 mRNA相对表达水平分别为1.00±0.04、0.58±0.05、1.00±0.08、0.54±0.07和0.29±0.03。miR-508-3p mimic组的上述指标与NC mimic组比较,si-ZEB1组的上述指标与共转染组比较,在统计学上差异均有统计学意义(P<0.01,P<0.05)。结论miR-508-3p在EOC癌组织中低表达,其可能通过靶控ZEB1表达,从而抑制卵巢癌细胞的迁移和侵袭。Objective To investigate the expression of microRNA-508-3p(miR-508-3p)in epithelial ovarian cancer(EOC)tissue,its impact on the migration and invasion of ovarian cancer cells,and its regulatory relationship with zinc-finger E-box-binding homeobox 1(ZEB1).Methods The surgical resection of EOC cancer tissues and paired adjacent normal tissues were collected.SKOv3 cells were divided into the NC mimic group(transfected with NC mimic),miR-508-3p mimic group(transfected with miR-508-3p mimic),si-NC group(transfected with si-NC),si-ZEB1group(transfected with si-ZEB1)and co-transfection group(co-transfected with si-ZEB1 and miR-508-3p mimic).The mRNA expression levels of miR-508-3p and ZEB1 in EOC cancer tissues,adjacent normal tissues and five groups of cells were measured by real-time quantitative polymerase chain reaction.The Transwell assay was used to detect the cell migration and invasion abilities.Results The relative expression levels of miR-508-3p in E0C tissues and adjacent normal tissues were 0.77±0.36 and 1.07±0.40,the relative expression levels of ZEB1 mRNA in EOC tissues and adjacent normal tissues were 2.10±1.21 and 1.29±0.95,and the differences were statistically significant(all P<0.01).The migration cell number of the NC mimic,miR-508-3p mimic,si-NC,si-ZEB1 and co-transfection groups was 633.00±32.49,319.20±19.89,650.40±25.85,375.00±17.25 and 129.40±17.10;the invasion cell number was 527.20±25.01,288.60±16.68,520.00±25.83,293.40±18.37 and 76.60±8.76;the relative expression levels of miR-508-3p were 1.05±0.37,3.94±1.21,1.01±0.21,1.26±0.34 and 3.40±0.41;the relative expression levels of ZEB1 mRNA were 1.00±0.04,0.58±0.05,1.00±0.08,0.54±0.07 and 0.29±0.03,respectively.The above indicators showed statistically significant dfferences between the miR-508-3p mimic group and the NC mimic group,between the si-NC group and the co-transfection group(P<0.01,P<0.05).Conclusion MiR-508-3p is lowly expressed in EOC cancer tissue,and it may inhibit the migration and invasion of ovarian cancer cel

关 键 词：微小RNA-508-3p 锌指E盒结合蛋白1 上皮性卵巢癌 迁移 侵袭 

分 类 号：R97[医药卫生—药品]