白藜芦醇介导miR-155调控SIRT1抑制脓毒症大鼠心肌损伤的研究  

Resveratrol mediates miR-155 regulation of SIRT1 to inhibit myocardial injury in sepsis rats

作　　者：卢晓航 高媛媛 李晓娟 胡雪莲 王玉巧[1] LU Xiao-hang;GAO Yuan-yuan;LI Xiao-juan;HU Xue-lian;WANG Yu-qiao(Department of Critical Care Medicine,Ningxia People's Hospital Affiliated to Ningxia Medical University,Yinchuan 750021,Ningxia Hui Autonomous Region,China)

机构地区：[1]宁夏医科大学附属宁夏人民医院重症医学科,宁夏回族自治区银川750021

出　　处：《中国临床药理学杂志》2025年第2期209-214,共6页The Chinese Journal of Clinical Pharmacology

基　　金：宁夏自然科学基金资助项目(2019AAC03304)。

摘　　要：目的探究白藜芦醇对由脓毒症导致心肌损伤大鼠的保护作用。方法将40只SD大鼠随机分成对照组、模型组、实验组和联合组,每组10只。除对照组外,其余大鼠腹腔注射20mg·kg^(-1)脂多糖造模,对照组注射等量0.9%NaCl;造模前2h,实验组和联合组均灌胃给予50mg·kg^(-1)白藜芦醇,对照组和模型组灌胃均给予等量0.9%NaCl;联合组经尾静脉注射微小RNA-155(miR-155)激动药10nmol,其余组大鼠经尾静脉注射等体积0.9%NaCl。用心脏超声检测大鼠左心室功能参数,用比色法检测心肌损伤标志物水平,用实时荧光定量聚合酶链反应检测miR-155相对表达水平,用蛋白质印迹法检测沉默信息调节因子1(SIRT1)和核因子κB信号通路相关蛋白的表达水平。结果对照组、模型组和实验组的左心室射血分数分别为(80.78±12.85)%、(55.92±7.86)%和(71.55±10.71)%,左心室缩短分数分别为(34.08±5.75)%、(22.92±2.96)%、(28.72±4.25)%,左心室收缩期内径分别为(3.12±0.46)、(6.34±0.69)和(4.95±0.57)mm,左心室舒张末内径分别为(5.98±0.65)、(7.24±0.80)和(6.16±0.78)mm,短轴缩短率分别为(38.91±5.38)%、(22.67±3.53)%和(30.74±3.97)%,肌酸激酶同工酶的表达水平分别为(661.56±85.44)、(1181.41±142.14)和(915.02±105.19)U·L^(-1),钙蛋白Ⅰ的表达水平分别为(148.17±28.48)、(448.17±60.34)和(375.44±49.01)ng·mL^(-1)。对照组、模型组、实验组和联合组的miR-155相对表达水平分别为1.00±0.12、3.79±0.45、1.87±0.23和4.03±0.49,核因子κB蛋白相对表达水平分别为1.00±0.08、5.04±0.59、2.73±0.35和5.58±0.63,核因子κB抑制激酶β蛋白相对表达水平分别为1.00±0.11、3.03±0.37、1.35±0.15和2.89±0.34,核因子κB抑制蛋白α蛋白相对表达水平分别为1.00±0.13、0.86±0.08、1.21±0.18和0.77±0.09,SIRT1蛋白相对表达水平分别为1.00±0.16、0.66±0.07、0.93±0.14和0.54±0.06。模型组的上述指标与对照组比较,实验组的上述指标与模Objective To explore the protective effect of resveratrol on myocardial damage caused by sepsis in rats.Methods Forty SD rats were randomly divided into control group,model group,experimental group and combined group,with 10 rats in each group.Except the control group,the other rats were intraperitoneally injected with 20 mg·kg^(-1)lipopolysaccharide,while the control group was injected with the same amount of normal saline.Two hours before modeling,the experimental group and combinated group were given 50 mg·kg^(-1)resveratrol by gavage,and the control group and model group were given the same amount of normal saline by gavage.The combined group was injected with 10 nmol of micro RNA-155(miR-155)agomir through the tail vein,and the other group was injected with equal volume of normal saline through the tail vein.Left ventricular function parameters of rats were measured by echocardiography.The level of myocardial injury markers was detected by colorimetry.Quantitative reverse transcription polymerase chain reaction was used to detect the expression of miR-155.The expression of sirtuin 1(SIRT1)and related proteins of nuclear factor kB signaling pathway were detected by Western blot.Results The left ventricular ejection fraction of control group,model group and experimental group were(80.78±12.85)%,(55.92±7.86)%and(71.55±10.71)%,respectively;left ventricular fractional shortening were(34.08±5.75)%,(22.92±2.96)%,(28.72±4.25)%,respectively;left ventricular end disatolic diameter were(3.12±0.46),(6.34±0.69),(4.95±0.57)mm,respectively;the left ventricular end systolic diameter were(5.98±0.65),(7.24±0.80),(6.16±0.78)mm,respectively;the fractional shortening were(38.91±5.38)%,(22.67±3.53)%,(30.74±3.97)%,and the expression levels of creatine kinase-MB were(661.56±85.44),(1181.41±142.14),(915.02±105.19)U L^(-1),respectively;the expressions levels of cardiac troponin I were(148.17±28.48),(448.17±60.34)and(375.44±49.01)ng·mL^(-1),respectively.The expression of miR-155 in control group,model group,exp

关 键 词：白藜芦醇 脓毒症 心肌损伤 炎症 沉默信息调节因子 

分 类 号：R28[医药卫生—中药学]