HIV感染患者单核细胞miR-221、miR-29及CXCL8、FOXP3表达水平  

Expressions of monocyte miR-221,miR-29,CXCL8 and FOXP3 in patients with HIV infection

作　　者：向龙春 周莹荃 宋小强 陈丽媛 王艳 梁惠霞 马小华 XIANG Long-chun;ZHOU Ying-quan;SONG Xiao-qiang;CHEN Li-yuan;WANG Yan;LIANG Hui-xia;MA Xiao-hua(Jiuquan Second People's Hospital,Jiuquan,Gansu 735000,China;不详)

机构地区：[1]酒泉市第二人民医院感染性疾病科,甘肃酒泉735000 [2]酒泉市第二人民医院耳鼻咽喉科,甘肃酒泉735000 [3]兰州市肺科医院感染性疾病科,甘肃兰州730000

出　　处：《中华医院感染学杂志》2025年第1期45-49,共5页Chinese Journal of Nosocomiology

基　　金：甘肃省卫生健康委科研项目(A2023010)。

摘　　要：目的探讨人类免疫缺陷病毒(HIV)感染患者单核细胞微小核糖核酸(miR)-221、miR-29及CXC趋化因子配体8(CXCL8)、叉头状转录因子P3(FOXP3)表达水平,为HIV感染的诊断提供理论参考。方法选取2020年11月-2023年12月酒泉市第二人民医院收治的118例HIV感染患者为感染组,选取同期到本院体检且与患者一般资料匹配的健康人群121名为健康组。比较两组外周血T淋巴细胞亚群(CD_(3)^(+)、CD_(4)^(+)、CD_(8)^(+)、CD_(4)^(+)/CD_(8)^(+))、miR-221、miR-29及CXCL8、FOXP3表达水平,分析miR-221、miR-29、CXCL8、FOXP3表达水平与HIV病毒载量的相关性,受试者工作特征(ROC)曲线分析miR-221、miR-29、CXCL8、FOXP3表达对HIV感染的诊断价值。结果感染组CD_(8)^(+)细胞数(598.79±73.92)个/μl高于健康组,CD_(3)^(+)细胞数(1184.94±129.75)个/μl、CD_(4)^(+)细胞数(586.15±83.22)个/μl、CD_(4)^(+)/CD_(8)^(+)(0.98±0.14)低于健康组(P<0.05)。感染组miR-221、miR-29、CXCL8表达水平高于健康组,FOXP3表达水平低于健康组(P<0.05)。miR-221、miR-29、CXCL8表达水平与HIV病毒载量呈正相关,FOXP3表达水平与HIV病毒载量呈负相关(P<0.05)。miR-221、miR-29、CXCL8、FOXP3联合诊断HIV感染的曲线下面积(AUC)值为0.947,高于单独检测(P<0.05),联合检测的敏感度为89.83%,特异度为91.74%。结论HIV感染不仅与T淋巴细胞亚群,还与miR-221、miR-29、CXCL8、FOXP3水平变化有关,且miR-221、miR-29、CXCL8、FOXP3表达水平与HIV病毒载量具有相关性,四者联合检测对HIV感染的诊断价值更高。OBJECTIVE To explore the expression levels of monocyte microribonucleic acid(miR)-221,miR-29,CXC chemokine ligand 8(CXCL8),forkhead transcription factor P3(FOXP3)in the patients with human immunodeficiency virus(HIV).so as to provide theoretical bases for diagnosis of HIV infection.METHODS Totally 118 patients with HIV infection who were treated in Jiuquan Second People's Hospital from Nov.2020 to Dec.2023 were assigned as the infection group,meanwhile,121 healthy people who received physical examination and of whom the baseline data were matched with the patients'were chosen as the healthy group.The expression levels of peripheral blood T lymphocyte subsets(CD_(3)^(+),CD_(4)^(+),CD_(8)^(+),CD_(4)^(+)/CD_(8)^(+)),miR-221,miR-29,CXCL8 and FOXP3 were compared between the two groups.The association between the expression levels of miR-221,miR-29,CXCL8,FOXP3 and the viral load of HIV was observed.The values of miR-221,miR-29,CXCL8 and FOXP3 in diagnosis of HIV infection were analyzed by means of receiver operating characteristic(ROC)curves.RESULTS The level of CD_(8)^(+)of the infection group was(598.79±73.92)/μl,higher than that of the healthy group,while the levels of CD_(3)^(+),CD_(4)^(+)and CD_(4)^(+)/CD_(8)^(+)of the infection group were(1184.94±129.75)/μl,(586.15±83.22)/μl and(0.98±0.14),respectively,lower than those of the healthy group(P<0.05).The expression levels of miR-221,miR-29 and CXCL8 of the infection group were higher than those of the healthy group,while the expression level of FOXP3 of the infection group was lower than that of the healthy group(P<0.05).The expression levels of miR-221,miR-29 and CXCL8 were positively correlated with the viral load of HIV,while the expression level of FOXP3 was negatively correlated with the viral load of HIV(P<0.05).The area under the curve(AUC)of the joint detection of miR-221,miR-29,CXCL8 and FOXP3 was 0.947,higher than that of the single detection in diagnosis of HIV infection(P<0.05),and the sensitivity of the joint detection was 89.83%,with the specifi

关 键 词：人类免疫缺陷病毒 感染 微小核糖核酸-221 微小核糖核酸-29 CXC趋化因子配体8 叉头状转录因子P3 T淋巴细胞 

分 类 号：R512.91[医药卫生—内科学]