厚胞汤调节成纤维细胞防治宫腔粘连的作用机制研究  

Mechanism of Houbao Decoction in the prevention and treatment of intrauterine adhesions by regulating fibroblast

作　　者：骆诗灵 赵宏利[1] 赵小萱 杨汉铭 王涵芝 丁心逸 LUO Shiling;ZHAO Hongli;ZHAO Xiaoxuan;YANG Hanming;WANG Hanzhi;DING Xinyi(Department of TCM Gynecology,Hangzhou Hospital of Traditional Chinese Medicine,Hangzhou 310007,China;Department of TCM Gynecology,Hangzhou Dingqiao Hospital,Hangzhou 310044,China;Hangzhou Clinical Medical College,Zhejiang Chinese Medical University,Hangzhou 310053,China)

机构地区：[1]杭州市中医院中医妇科,杭州310007 [2]杭州市丁桥医院中医妇科,杭州310044 [3]浙江中医药大学杭州临床医学院,杭州310053

出　　处：《浙江中西医结合杂志》2025年第2期108-111,120,共5页Zhejiang Journal of Integrated Traditional Chinese and Western Medicine

基　　金：浙江省中医药科技计划项目(No.2023ZL513);浙江省公益基金联合项目(No.LBY24H040011);浙江中医药大学校级科研项目附属医院科研专项(No.2022FSYYZZ13)。

摘　　要：目的探讨厚胞汤调节成纤维细胞对防治宫腔粘连的作用机理。方法将10只健康SD大鼠随机数字表法分成空白对照组和厚胞汤组,空白对照组灌胃等量生理盐水,厚胞汤组灌胃厚胞汤2 m L/只·次,每天2次以制备空白血清及含药血清,将经转化生长因子-β1(TGF-β1)刺激后的成纤维细胞分为空白对照组和厚胞汤组,采用CCK8法检测两组细胞增殖情况,流式细胞法检测细胞凋亡水平、细胞周期变化,Western blot检测两组细胞的TGF-β1、信号转导蛋白家族(Smads)表达水平。结果与空白对照组比较,厚胞汤组的细胞增殖率显著降低[第2天:(1.05±0.04)%比(1.19±0.02)%,P<0.01;第3天:(1.11±0.02)%比(1.36±0.02)%,P<0.01;第4天:(1.18±0.02)%比(1.55±0.02)%,P<0.001;第5天:(1.16±0.02)%比(1.89±0.03)%,P<0.01];细胞凋亡率显著升高[(10.19±0.72)%比(6.31±0.17)%,P<0.01];细胞周期G1期比例降低[(48.78±1.51)%比(52.30±0.56)%,P<0.05],G2期比例升高[(27.56±0.64)%比(22.86±0.88)%,P<0.01]。Western blot结果显示,厚胞汤组TGF-β1、Smad3表达显著下调。结论厚胞汤可能通过TGF-β/Smads通路有效抑制成纤维细胞纤维化表达,从而对宫腔粘连起到防治作用。Objective To investigate the mechanism of Houbao Decoction in the prevention and treatment of intrauterine adhesions by regulating fibroblast.Methods Ten healthy SD rats were randomly divided into a control group and a Houbao Decoction group using a random number table.The Houbao Decoction group was gavaged with 2 mL per rat per dose of Houbao Decoction,twice a day,while the control group was gavaged with an equal amount of physiological saline.Blank serum and drug-containing serum were collected.The fibroblast stimulated by transforming growth factor-β1(TGF-β1)were divided into a control group and a Houbao Decoction group.The cell proliferation in both groups was detected using the CCK8 method.Apoptosis and cell cycle were analyzed using flow cytometry.The expression levels of TGF-β1 and the signal transduction protein family(Smads)in both groups were detected using western blotting.Results Compared with the control group,the cell proliferation rate in the Houbao Decoction group was significantly reduced[Day 2:(1.19±0.02)vs.(1.05±0.04)%,P<0.01;Day 3:(1.36±0.02)vs.(1.11±0.02)%,P<0.001;Day 4:(1.55±0.02)vs.(1.18±0.02)%,P<0.001;Day 5:(1.89±0.03)vs.(1.16±0.02)%,P<0.001].The cell apoptosis rate was significantly increased[(10.19±0.72)vs.(6.31±0.17)%,P<0.01].The proportion of cells in the G1 phase of the cell cycle was decreased[(48.78±1.51)vs.(52.30±0.56)%,P<0.05],while the proportion of cells in the G2 phase was increased[(27.56±0.64)vs.(22.86±0.88)%,P<0.01].Conclusion Houbao Decoction may inhibit the fibrotic expression of fibroblast by the TGF-β/Smads pathway,thereby playing a role in the prevention and treatment of intrauterine adhesions.

关 键 词：大鼠 宫腔粘连 厚胞汤 成纤维细胞 转化生长因子-Β SMAD3 

分 类 号：R285.5[医药卫生—中药学]