铜钆纳米材料对大肠埃希菌的抗菌性能研究  

Study on the antibacterial properties of Copper-Gadolinium nanomaterials against Escherichia coli

作　　者：李青 司元纯 吴正岩 张嘉 王元银[1] Li Qing;Si Yuanchun;Wu Zhengyan;Zhang Jia;Wang Yuanyin(College&Hospital of Stomatology,Anhui Medical University,Anhui Provincial Key Laboratory of Oral Disease Research,Hefei 230032;Institute of Intelligent Machines,Hefei Institutes of Physical Science,Chinese Academy of Sciences,Hefei 230031)

机构地区：[1]安徽医科大学口腔医学院,安徽医科大学附属口腔医院,安徽省口腔疾病研究重点实验室,合肥230032 [2]中国科学院合肥物质科学研究院智能机械研究所,合肥230031

出　　处：《安徽医科大学学报》2024年第12期2157-2162,共6页Acta Universitatis Medicinalis Anhui

基　　金：安徽省重点研究和开发计划项目(编号:201904a07020062);安徽省卫生健康科研项目(编号:AHWJ2022 a012);安徽高校协同创新项目(编号:GXXT-2021-056);安徽医科大学校科研基金项目(编号:2020xkj022)。

摘　　要：目的探究铜钆纳米材料的生物安全性及其对大肠埃希菌(E.coli)的抗菌性能。方法采用水热法制备铜钆纳米材料,利用透射电子显微镜等仪器对样品进行表征分析;通过小鼠成纤维细胞(L929)的CCK-8实验评估铜钆纳米材料的生物安全性;将铜钆纳米材料与E.coli共孵育,观察并评估其抗菌性能。结果透射电镜及傅里叶红外检测分析结果确认了铜钆纳米材料的合成。CCK-8实验结果显示该材料在0~2μg/ml浓度范围内具有可靠的生物安全性。抗菌实验验证了铜钆纳米材料良好的抗E.coli活性,且抗菌效果与浓度和共孵育时间呈正相关,2μg/ml的铜钆纳米材料与E.coli共孵育4 h,其抗菌率达99.99%。结论2μg/ml铜钆纳米材料具有可靠的生物安全性和良好的抗E.coli能力。Objective To investigate the biocompatibility of copper-gadolinium nanomaterials and their antibacterial efficacy against Escherichia coli(E.coli).Methods Copper-gadolinium nanomaterials were prepared using a hydrothermal method,and the samples were characterized and analyzed using instruments such as transmission electron microscopy.The biocompatibility of copper-gadolinium nanomaterials was evaluated through CCK-8 experiments with mouse fibroblast cells(L929).Copper-gadolinium nanomaterials were co-cultured with E.coli,and their antibacterial performance was observed and assessed.Results Transmission electron microscopy and Fourier transform infrared spectroscopy analysis confirmed the synthesis of the copper-gadolinium nanomaterials.The CCK-8 experiment results showed that the material had reliable biocompatibility within a concentration range of 0-2μg/ml.The antibacterial experiments verified the excellent anti-E.coli activity of copper-gadolinium,with the antibacterial effect positively correlating with concentration and co-cultivation time.When copper-gadolinium nanomaterials at a concentration of 2μg/ml were co-cultured with E.coli for 4 hours,the antibacterial rate reached 99.99%.Conclusion The 2μg/ml copper-gadolinium nanomaterials exhibit reliable biocompatibility and excellent anti-E.coli capabilities.

关 键 词：铜钆 纳米材料 核壳结构 抗菌 大肠埃希菌 小鼠成纤维细胞L929 

分 类 号：R378.21[医药卫生—病原生物学] O614.121[医药卫生—基础医学] O616[理学—无机化学]