μ-阿片受体在心内神经节的表达分布及其激动剂对房颤的作用  

Expression and distribution ofμ-opioid receptors in intracardiac ganglia and effects of its agonists on atrial fibrillation

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作  者:刘海帆 谢雨杉 万法萍 Liu Haifan;Xie Yushan;Wan Faping(Dept of Human Anatomy,Xuzhou Medical University,Xuzhou 221004)

机构地区:[1]徐州医科大学人体解剖学教研室,徐州221004

出  处:《安徽医科大学学报》2024年第12期2162-2169,共8页Acta Universitatis Medicinalis Anhui

基  金:国家自然科学基金项目(编号:81801104)。

摘  要:目的研究μ-阿片受体(MOR)在心内神经节(ICG)的表达分布,并探讨MOR激动剂对心房颤动(AF)的影响。方法①通过免疫荧光单标染色法研究正常雄性SD大鼠ICG的化学解剖学特性;采用免疫荧光双标染色、Western blot和RT-PCR法检测MOR在ICG的表达分布。②40只250~300 g SD雄性大鼠随机分为正常组(Normal组)、AF模型组(AF组)、溶剂对照组(AF-NS组)、MOR特异性激动剂内吗啡肽2(EM2)药物组(AF-EM2组)和DAMGO药物组(AF-DAMGO组),采用尾静脉注射乙酰胆碱-氯化钙混合液方法制备AF模型,造模同时给予药物干预;采集心电图并监测给药前后AF持续时间,采用Western blot和qRT-PCR法检测各组大鼠ICG中MOR以及心房组织提取物中连接蛋白43(CX43)的蛋白及mRNA表达。结果①ICG中含有乙酰胆碱转移酶阳性的副交感神经元、酪氨酸羟化酶阳性的交感神经元以及P物质和降钙素基因相关肽阳性的肽能神经纤维;心房后壁组织表达MOR mRNA转录物和蛋白;MOR免疫阳性产物主要分布于ICG神经元的胞体内,且以副交感和交感神经元为主。②与Normal组相比,AF组大鼠ICG中MOR mRNA以及蛋白表达量均降低(P<0.05);与AF-NS组相比,AF-EM2组和AF-DAMGO组AF持续时间均缩短,CX43表达量均升高(P<0.05)。结论MOR主要表达于ICG的交感和副交感神经元中;AF大鼠ICG中MOR表达量降低,MOR激动剂能缓解AF。Objective To explore the expression and distribution ofμ-opioid receptors(MOR)in intracardiac ganglia(ICG)and the effect of its agonists on atrial fibrillation(AF).Methods①The chemical anatomical characteristics of ICG in normal SD rats were studied by immunofluorescence single staining.The expression and distribution of MOR in ICG were detected by using immunofluorescence double staining,Western blot,and RT-PCR.②Forty male SD rats weighing 250-300 g were randomly divided into 5 groups:normal group,AF model group(AF group),solvent control group(AF-NS group),MOR-specific agonist endomorphin-2(EM2)drug group(AF-EM2 group)and DAMGO drug group(AF-DAMGO group).The AF model was established by tail vein injection of acetylcholine(Ach)and CaCl 2,and drug intervention was given during modeling.The duration of AF before and after drug administration was monitored by collecting electrocardiograms.The protein and mRNA expression of MOR in ICG and connexin 43(CX43)in atrial tissue were detected by the method of Western blot and qRT-PCR.Results①Peptide nerve fibers positive for substance P and calcitonin gene-related peptide were found in the ICG,along with parasympathetic neurons positive for acetylcholine transferase and sympathetic neurons positive for tyrosine hydroxylase.The mRNA transcripts and protein of MOR were expressed in the atrial posterior wall tissue.MOR immunoreactive products were mainly distributed in the cell bodies of ICG neurons,primarily in parasympathetic and sympathetic neurons.②Compared with the Normal group,the expression of MOR mRNA and protein in ICG of the AF rat decreased(P<0.05).Compared with the AF-NS group,the duration of AF was shortened in the AF-EM2 group and AF-DAMGO group,and the expression of CX43 increased(P<0.05).Conclusion MOR is mainly expressed in sympathetic and parasympathetic neurons in ICG.The expression of MOR decreases in AF rats.MOR agonists alleviate AF.

关 键 词:Μ-阿片受体 心内神经节 房颤 μ-阿片受体激动剂 CX43 

分 类 号:R322.85[医药卫生—人体解剖和组织胚胎学]

 

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