自适应精准控温型液相色谱柱温箱的设计与评价  

Design and evaluation of a liquid chromatographic column oven with adaptive and precise temperature control

作　　者：任兴发 邵锋伟 吴勇 廖信明 王卓[1,2] 周林娟 何旭鸿 张维冰 REN Xingfa;SHAO Fengwei;WU Yong;LIAO Xinming;WANG Zhuo;ZHOU Linjuan;HE Xuhong;ZHANG Weibing(East China University of Science and Technology,Shanghai 200237,China;Welch Technology(Shanghai)Co.Ltd.,Shanghai 201613,China;Welch Materials(Zhejiang),Inc.,Jinhua 321000,China)

机构地区：[1]华东理工大学,上海200237 [2]月旭科技(上海)股份有限公司,上海201613 [3]浙江月旭材料科技有限公司,浙江金华321000

出　　处：《色谱》2025年第2期177-184,共8页Chinese Journal of Chromatography

基　　金：国家重点研发计划资助项目(2021YFF0701900);上海市2020年度“科技创新行动计划”科学仪器领域项目(20142200900).

摘　　要：高效液相色谱系统作为诸多领域的关键分析手段,提升其分离效率、稳定性和普适性是研究重点。在该技术手段中,流动相的组成和比例、固定相的种类以及色谱柱的尺寸等显著影响分离效果,色谱柱和流动相的温度对分离效果也起到了至关重要的作用。高效液相色谱系统通常采用柱温箱进行温度控制,高精度的温度控制可提升色谱分离性能、缩短分析时间、确保分析结果的重复性。本文创新性地改进了柱温箱的结构,通过融合多种先进温度控制算法,实现了高精度、宽范围(4~90℃)的连续升降温控制,为色谱分析方法发展开辟了新路径。此改进摒弃了复杂的硬件系统,仅通过软件优化即达成了高度稳定的控温效果,控温精度提升至±0.1℃。此外,通过优化保温结构与采用环保隔热材料,也增强了控温精细化能力;对于热源热传导机制的研究,进一步提升了柱温箱的综合性能。结果表明,该改进显著提高了色谱分离的重复性和稳定性,为高效色谱分析方法的发展奠定了坚实基础。High performance liquid chromatography(HPLC)is a key analytical technique that is used in a number of fields.Improving the separation efficiency,stability,and universality of HPLC has been a continuing analytical-chemistry focus.In chromatographic separation,factors such as the composition and ratio of the mobile phase,the type of stationary phase,and the dimensions of the chromatographic column significantly affect the separation efficiency.In addition,the temperatures of the chromatographic column and mobile phase are also important for achieving separation.The column oven is usually used to stably control the column temperature in the HPLC separation system.Indeed,highly accurate temperature control ensures superior separation performance,short analysis times,and repeatability.In this study,we innovatively improved the traditional column oven by combining a variety of temperature-control algorithms to deliver continuous and highly accurate temperature control in the wide 4-90℃range,and by exploring a new chromatographic-method development route.Instead of focusing on the complex hardware system,we optimized the software to achieve highly stable and accurate(±0.1℃)temperature control.Temperature-control performance was further improved by optimizing the structure of the thermal insulation and employing reliable and environmentally friendly thermal-insulation materials.Additionally,the thermal conduction of the heat-source device is discussed based on the heat-transfer principle with the aim of improving the performance of the column oven.The improved column oven delivered significantly enhanced chromatographic-separation repeatability and stability thereby reliably guaranteeing the development of highly efficient chromatographic analysis methods.

关 键 词：精准控温 算法 传热原理 液相色谱 柱温箱 

分 类 号：O658[理学—分析化学]