山西谷子萌发期耐盐性鉴定及相关性状GWAS分析  

Salt Tolerance Identification and GWAS Analysis of Shanxi Foxtail Millet during Germination Stage

作　　者：秦慧彬 任觐 娄坤 商春悦 阳世杰 侯森 李萌 王海岗[1] 穆志新 QIN Huibin;REN Jin;LOU Kun;SHANG Chunyue;YANG Shijie;HOU Sen;LI Meng;WANG Haigang;MU Zhixin(Center for Agricultural Genetic Resources Research,Shanxi Agricultural University/Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau,Ministry of Agriculture and Rural Affairs,Taiyuan 030031;College of Agronomy,Shanxi Agricultural University,Taigu 030801)

机构地区：[1]山西农业大学农业基因资源研究中心/农业农村部黄土高原作物基因资源与种质创制重点实验室,太原030031 [2]山西农业大学农学院,太谷030801

出　　处：《植物遗传资源学报》2025年第1期108-119,共12页Journal of Plant Genetic Resources

基　　金：国家农作物种质资源保护与利用项目(19230853);山西省农作物种质资源保护与利用项目(sxzyk202301);国家农作物种质资源平台项目(NCGRC-2023-026);山西省种业创新良种联合攻关(gzlg-02-03)。

摘　　要：为研究山西谷子萌发期耐盐性、筛选与谷子耐盐性相关的候选基因,及了解谷子种子在盐胁迫下萌发相关性状的遗传机制,本试验共抽取333份山西核心种质,在光照培养室采用170 mmol/L NaCl溶液胁迫,对萌发期谷子进行耐盐性鉴定。采用隶属函值法对谷子耐盐等级进行分类,以相对发芽势、相对发芽率、相对根长、相对芽长、相对发芽指数5个相关性状的综合评价D值对谷子萌发期耐盐性进行评价。结合核心种质重测序结果,开展耐盐性全基因组在萌发期的关联分析。结果表明,供试谷子核心种质耐盐性差异显著,利用综合评价D值,把333份谷子核心种质的耐盐性划分为5类,其中高耐盐种质有17份。全基因组关联分析共检测到84个与萌发期耐盐性相关的SNP位点,对显著关联SNPs所在基因进行功能注释,分析筛选到与萌发期相关的耐盐候选基因Millet_GLEAN_10019153,并对该基因进行了单倍型分析。本研究结果对耐盐谷子种质的鉴选及耐盐基因挖掘具有一定参考意义。In order to investigate the salt tolerance of Shanxi foxtail millet during germination,identify candidate genes associated with this trait,and elucidate the genetic mechanisms underlying germination traits under salt stress,a total of 333 core germplasms from Shanxi,China,were selected for assessing salt tolerance during germination.A stress of 170 mmol/L NaCl stress treatment was applied in a controlled light chamber to determine the salt tolerance during germination.The subordinate function value method was used to categorize the salt tolerance grades of the millets,and a comprehensive evaluation D value of five related traits,namely,relative germination potential,relative germination rate,relative root length,relative shoot length,and relative germination index,was used to access salt tolerance at the germination stage.In conjugation with the resequencing results of core germplasm,a genome-wide association scanning(GWAS)for salt tolerance at the germination stage was carried out.The findings revealed significant variation in salt tolerance in the collection,which were classified into five categories using the comprehensive evaluation D-value,with 17 exhibiting high salt tolerance.GWAS identified 84 single nucleotide polymorphism(SNP)loci associated with salt tolerance during emergence.Functional annotation was performed on the genes where the significantly associated SNPs were located.This led to the identification of the salt tolerance candidate gene Millet_GLEAN_10019153,which is associated with emergence.The outcomes of this study offer valuable references for the identification of salt-tolerant cereal germplasm and the mining of salt-tolerant genes.

关 键 词：谷子 核心种质 萌发期 耐盐性 GWAS 

分 类 号：S515[农业科学—作物学]