番茄叶色基因Sllc1精细定位及候选基因分析  

Fine-mapping and Candidate Gene Analysis of Tomato Leaf Color Gene Sllc1

作　　者：阮美颖[1] 柴亚倩 周国治[1] 王荣青[1] 叶青静[1] 万红建[1] 姚祝平[1] 李志邈[1] 程远[1] RUAN Meiying;CHAI Yaqian;ZHOU Guozhi;WANG Rongqing;YE Qingjing;WAN Hongjian;YAO Zhuping;LI Zhimiao;CHENG Yuan(Institute of Vegetables,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021;College of Agriculture,Shihezi University,Shihezi 832003,Xinjiang)

机构地区：[1]浙江省农业科学院蔬菜研究所,杭州310021 [2]石河子大学农学院,新疆石河子832003

出　　处：《植物遗传资源学报》2025年第1期177-188,共12页Journal of Plant Genetic Resources

基　　金：浙江省农业(蔬菜)新品种选育重大科技专项(2021C02065);国家自然科学基金(32341044);国家现代农业产业技术体系(CARS-23-G44);浙江省重点研发计划项目(2024C02003,2021C02052)。

摘　　要：叶色突变体作为厘清植物叶绿素生物合成及叶绿体发育机制的重要工具,对于探究植物生长发育进程具有重要意义。本研究以绿色叶片番茄材料CR11A和浅黄色叶片番茄材料CH09-805为亲本,通过构建遗传群体明确叶色的遗传规律;对不同叶色植株开展叶片叶绿体超微结构观测及叶绿素含量测定,并进一步利用BSA-Seq及分子标记进行叶色基因定位,开展候选基因分析。研究结果表明,F_(1)植株叶片为绿色、F_(2)群体植株叶片出现12(绿色)∶3(浅黄色)∶1(金黄色)的分离比,说明番茄叶色性状受两对基因控制且存在显性上位效应;通过叶绿体超微结构观测及叶绿素含量测定,发现金黄色叶片叶绿体超微结构严重受损,其叶绿素含量显著低于浅黄色叶片与绿色叶片;利用BSA-Seq及分子标记将番茄叶色基因Sllc1定位于7号染色体114.53 kb的物理距离,候选区间内包括13个注释基因,结合基因注释信息及表达量鉴定,推测Solyc07g053630与Solyc07g053640为Sllc1的候选基因。本研究获得番茄叶色的候选基因,为番茄叶色形成分子机制解析奠定重要的材料基础及基因资源。Leaf color mutants are valuable tools for elucidating the mechanisms of chlorophyll biosynthesis and chloroplast development,and they play a significant role in understanding plant growth and development processes.In this study,green-leafed tomato material CR11A and yellow-leafed tomato material CH09-805 were used as parents to construct a genetic population to clarify the genetic rules of leaf color.Leaf chloroplast ultrastructure observation and chlorophyll content determination were performed on plants with different leaf colors.Furthermore,BSA-Seq and molecular marker screening were used for leaf color gene mapping and candidate gene analysis.The results showed that the F_(1) plants had green leaves,and the F_(2) population exhibited a segregation ratio of 12(green)∶3(yellow)∶1(golden),indicating that tomato leaf color traits are controlled by two pairs of genes with dominant epistasis.Observation of chloroplast ultrastructure and determination of chlorophyll content revealed that the chloroplast ultrastructure of golden yellow leaves was severely damaged,and their chlorophyll content was significantly lower than that of pale yellow and green leaves.Using BSA-Seq and molecular marker screening,the tomato leaf color gene Sllc1 was mapped to a 114.53 kb physical distance on chromosome 7.The candidate interval included 13 annotated genes.Based on gene annotation information and expression identification,Solyc07g053630 and Solyc07g053640 were identified as candidate genes for Sllc1.This study identified candidate genes for tomato leaf color,providing important material and gene resources for elucidating the molecular mechanisms of tomato leaf color formation.

关 键 词：番茄 叶色 BSA-Seq 分子标记 候选基因 

分 类 号：S641.2[农业科学—蔬菜学]