两段式PN-SAED工艺实现低碳氮比污水深度脱氮  

Achieving Deep Nitrogen Removal via Two‑stage PN-SAED Process from Low C/N Ratio Wastewater

作　　者：刘文龙 应乾鸿 王佳铭 孙丽娜 李军 LIU Wen‑long;YING Qian‑hong;WANG Jia‑ming;SUN Li‑na;LI Jun(Key Laboratory of Microbial Technology for Industrial Pollution Control of Zhejiang Province,College of Environment,Zhejiang University of Technology,Hangzhou 310014,China)

机构地区：[1]浙江工业大学环境学院浙江省工业污染微生物控制技术重点实验室,浙江杭州310014

出　　处：《中国给水排水》2025年第1期1-8,共8页China Water & Wastewater

基　　金：浙江省“领雁”研发攻关计划项目(2022C03075);浙江省基础公益研究计划项目(LQ21E080013);国家自然科学基金资助项目(52000157)。

摘　　要：为解决城市污水主流厌氧氨氧化工艺出水硝态氮积累且易失稳的问题,构建了两段式短程硝化-厌氧氨氧化耦合内源反硝化(PN-SAED)工艺,考察了其对低碳氮比(C/N)污水的深度脱氮性能和微生物菌群结构变化。在进水C/N为2.60±0.11时,PN-SAED工艺的出水总无机氮(TIN)浓度为(2.6±0.5)mg/L,TIN去除率达到(94.6±1.0)%。典型周期的氮素平衡分析表明,PN和SAED系统对氮素的去除贡献分别为7.3%和92.7%,其中SAED系统中氮素主要通过厌氧氨氧化途径去除(贡献率达82.2%)。高通量测序数据表明,投加羟胺可在实现PN系统中亚硝酸盐氧化菌属丰度(Nitrospira,3.1%→0.6%)快速下降的同时,使氨氧化菌属(Nitrosomonas,0.13%→0.92%)得到富集,实现稳定短程硝化;而在SAED系统中Candidatus Brocadia(12.3%)和Candidatus Jettenia(2.83%)成为优势厌氧氨氧化菌属,与内源反硝化菌属Candidatus Competibacter(17.5%)和Denitratisoma(7.15%)协同共生,从而实现对低C/N城市污水的深度脱氮。To address the issue of instability and nitrate accumulation in the effluent of the mainstream anaerobic ammonia oxidation(Anammox)process treating municipal wastewater,this study established a two‑stage process combining partial nitrification/Anammox with endogenous denitrification(PN-SAED)process for low carbon to nitrogen(C/N)ratio wastewater treatment,and the deep nitrogen removal performance and changes in microbial community structure were evaluated.The results indicated that,with an influent C/N ratio of 2.60±0.11,the PN-SAED process achieved an effluent total inorganic nitrogen(TIN)concentration of(2.6±0.5)mg/L,corresponding to a TIN removal efficiency of(94.6±1.0)%.Nitrogen balance analysis during a typical cycle showed that the contributions of the PN and SAED systems to nitrogen removal were 7.3%and 92.7%,respectively,with the Anammox pathway accounting for the majority of nitrogen removal(82.2%)in the SAED system.High‑throughput sequencing data revealed that the addition of hydroxylamine led to a rapid decline in the abundance of nitriteoxidizing bacteria(Nitrospira,from 3.1%to 0.6%)in the PN system,while enriching ammonia‑oxidizing bacteria(Nitrosomonas,from 0.13%to 0.92%),achieving stable partial nitrification.In the SAED system,Candidatus Brocadia(12.3%)and Candidatus Jettenia(2.83%)emerged as the dominant Anammox bacteria,coexisting synergistically with endogenous denitrifying bacteria of Candidatus Competibacter(17.5%)and Denitratisoma(7.15%),thereby accomplishing deep nitrogen removal from municipal wastewater with a low C/N ratio.

关 键 词：短程硝化 厌氧氨氧化 内源反硝化 深度脱氮 菌群结构 

分 类 号：TU992[建筑科学—市政工程]