电针通过前扣带回脑区谷氨酸能神经元缓解面神经损伤小鼠焦虑样行为  

Electroacupuncture alleviates facial nerve injury-induced anxiety-like behavior by raising the excitability of glutamatergic neurons in the anterior cingulate cortex

作　　者：张芷晴 申晨薇 臧晓宇 徐文资 姬昌 张文捷 王聪[2] 王琳 刘健华[2] ZHANG Zhi-qing;SHEN Chen-wei;ZANG Xiao-yu;XU Wen-zi;JI Chang;ZHANG Wen-jie;WANG Cong;WANG Lin;LIU Jian-hua(South China Research Center for Acupuncture and Moxibustion,Clinical Medical College of Acupuncture and Rehabilitation,Guangzhou University of Chinese Medicine,Guangzhou 510006,China;Guangdong Provincial Hospital of Traditional Chinese Medicine,Guangzhou 510120;Basic Medical Science College,Guangzhou University of Chinese Medicine,Guangzhou 510006;The First Affiliated Hospital,Jinan University,Guangzhou 510630)

机构地区：[1]广州中医药大学针灸康复临床医学院华南针灸研究中心,广州510006 [2]广东省中医院,广州510120 [3]广州中医药大学基础医学院,广州510006 [4]暨南大学第一附属医院,广州510630

出　　处：《针刺研究》2025年第1期14-21,共8页Acupuncture Research

基　　金：国家自然科学基金项目(No.82074544);广东省中医药管理局科研项目(No.20241077);广州市科技局科研项目(No.2024A04J4982);大学生创新创业训练计划项目(No.202410572058)。

摘　　要：目的:探讨电针能否缓解面神经损伤小鼠的焦虑样行为,以及电针的效应是否与前扣带回皮层(anterior cingulate cortex,ACC)谷氨酸能神经元有关。方法:30只C57BL/6J成年雄性小鼠随机分为对照组、模型组、电针组,每组10只。钳夹面神经干法制作单侧面神经挤压伤模型。造模21 d后,电针组小鼠电针“四关”穴干预30 min,连续7 d,对照组和模型组小鼠给予同样麻醉,但不进行电针干预。以明暗箱实验、高架十字迷宫实验评估小鼠焦虑样行为;光纤钙成像技术观察ACC脑区谷氨酸能神经元的活性;全细胞膜片钳技术记录ACC脑区谷氨酸能神经元内在兴奋性的水平。结果:与对照组相比,模型组小鼠在明暗箱的明箱逗留时间及穿梭次数均减少(P<0.01),在高架十字迷宫实验的开放臂停留时间及进入开放臂的次数均减少(P<0.01);在开放臂期间ACC脑区谷氨酸能神经元的活性显著降低(P<0.05);ACC脑区谷氨酸能神经元输入阻抗显著降低(P<0.05),在300 pA时放电数目减少(P<0.01)。与模型组相比,电针组小鼠在明箱逗留时间及穿梭次数均增加(P<0.01),在开放臂停留的时间及进入开放臂的次数均增加(P<0.01,P<0.05);在开放臂期间ACC脑区谷氨酸能神经元的活性显著增强(P<0.05);ACC脑区谷氨酸能神经元输入阻抗显著升高(P<0.05),在300 pA时放电数目显著增加(P<0.05)。结论:电针可有效缓解面神经损伤小鼠焦虑样行为,其机制可能是通过提高由于面神经损伤减弱的ACC脑区中谷氨酸能神经元兴奋性水平实现的。Objective To investigate the effect and mechanism of electroacupuncture(EA)at"Siguan"acupoints(bilateral Hegu[LI4]and bilateral Taichong[LR3])inamelioratingfacial nerveinjury-induced anxiety-like behavior in mice.Methods Thirty male C57BL/6J mice were randomly divided into control,model(unilateral facial nerve injury),and EA groups,with 10 mice in each group.The facial nerve injury model was established by lightly clamping the isolated facial nerve for about 3o s,twice altogether after operation.The mice of the EA group were subjected to EA stimulation(2 Hz,0.5 mA)of bilateral LI4 and bilateral LR3 for 30 min,once per day for consecutive 7 d.The anxiety-like behavior of mice was evaluated by light-dark chamber(LDC)and elevated plus maze(EPM)tests after EA intervention.The calcium activity of glutamatergic neurons in the anterior cingulate cortex(AcC)was analyzed by fiber photometry.The intrinsic excitability level of Acc glutamatergic neurons was estimated by using whole-cell patch clamp recording.Results Compared with the control group,the dwell time in the light chamber and the number of entries into the light chamber of LDC tests,and the dwell time in the open arms and the number of entries into the open arms of the EPM tests were significantly decreased in the model group(P<0.01).In comparison with the model group,the dwell time in the light chamber and the number of entries into the light chamber,and the dwell time in the open arms and the number of entries into the open arms were significantly increased in the EA group(P<0.01,P<0.05).Results of fiber photometry showed a decreased activity of ACC glutamatergic neurons when the mice moved from the closed arm to the open arm in an EPM,a decrease of input resistance and the number of discharge spikes induced by 300 pA current injection in the model group(P<0.05,P<0.01).Compared with the model group,the excitability of the glutamatergic neurons,input resistance and the number of discharge spikes(induced by 300 pA current injection)were considerably increased in

关 键 词：电针“ 四关”穴 焦虑样行为 面神经损伤 前扣带回皮层 谷氨酸能神经元 

分 类 号：R245.97[医药卫生—针灸推拿学]