电针“百会”“神庭”对血管性痴呆大鼠认知障碍和线粒体能量代谢的影响  

Effect of electroacupuncture at“Baihui"(GV20)and“Shenting"(GV24)on cognitive impairment and mitochondrial energy metabolism in vascular dementia rats

作　　者：贾晓钰 李惠菁 饶婷 游咏梅 李涓 龚一萌 钟冬灵 江一静 JIA Xiao-yu;LI Hui-jing;RAO Ting;YOU Yong-mei;LI Juan;GONG Yi-meng;ZHONG Dong-ling;JIANG Yi-jing(School of Health Preservation and Rehabilitation,Chengdu University of Traditional Chinese Medicine,Chengdu 610075,China;College of Clinical Medicine,Chengdu University of Traditional Chinese Medicine,Chengdu 610075;Rehabilitation Hospital Affiliated to Fujian University of Traditional Chinese Medicine,Fuzhou 350003,China)

机构地区：[1]成都中医药大学养生康复学院,成都610075 [2]成都中医药大学临床医学院,成都610075 [3]福建中医药大学附属康复医院,福州350003

出　　处：《针刺研究》2025年第1期22-31,共10页Acupuncture Research

基　　金：福建省自然科学基金项目(No.2020J01763、2021J01955);中医康复学科开放课题重点项目(No.XKF2023011);福建省卫生健康中青年领军人才研修培养项目(No.0040701040201)。

摘　　要：目的:探讨电针对血管性痴呆(VD)大鼠腺苷酸活化蛋白激酶(AMPK)信号通路及线粒体能量代谢的影响,阐释其防治VD的可能机制。方法:雄性SD大鼠随机分为假手术组、模型组、电针组、非经非穴组、电针+抑制剂组,每组8只,除假手术组外,其余各组采用双侧颈总动脉永久性结扎法建立VD模型。电针组电针“百会”“神庭”,非经非穴组电针双侧胁下髂嵴上10 mm处,疏密波2 Hz/15 Hz,30 min/次,1次/d,持续治疗7 d;电针+抑制剂组在电针干预前2 h腹腔注射AMPK抑制剂。干预后,Morris水迷宫实验检测大鼠学习记忆能力,ELISA法检测大鼠海马线粒体复合物Ⅰ—Ⅳ含量,比色法检测海马三磷酸腺苷(ATP)含量,透射电镜观察海马线粒体超微结构,Western blot法检测海马AMPKα1、脂肪酸转运酶(FAT)/分化簇36(CD36)、乙酰辅酶A羧化酶2(ACC2)蛋白表达,Pearson相关分析分析能量代谢标志物含量与行为学相关性。结果:与假手术组比较,模型组大鼠的逃避潜伏期与总路程增加(P<0.01),目标象限停留时间和穿越原平台次数减少(P<0.01),海马线粒体复合物Ⅰ—Ⅳ含量及ATP含量降低(P<0.01),线粒体结构重度破坏,海马AMPKα1、FAT/CD36蛋白表达降低(P<0.01),ACC2蛋白表达升高(P<0.01)。与模型组比较,电针组大鼠逃避潜伏期与总路程减少(P<0.01),目标象限停留时间和穿越原平台次数增加(P<0.01),海马线粒体复合物Ⅰ—Ⅳ含量及ATP含量升高(P<0.01),线粒体结构轻度异常,海马AMPKα1、FAT/CD36蛋白表达升高(P<0.01),ACC2蛋白表达降低(P<0.01),且改善程度优于非经非穴组与电针+抑制剂组(P<0.01)。相关性分析表明,线粒体复合物I含量与大鼠逃避潜伏期、总路程呈负相关(P<0.000 1),与目标象限停留时间、穿越原平台次数呈正相关(P<0.05, P<0.000 1)。结论:电针可能通过激活AMPK,上调FAT/CD36同时下调ACC2蛋白的表达,促进线粒体脂肪酸代谢,改善线粒体形态与功�Objective To observe the effect of electroacupuncture(EA)on adenosine monophosphate-activated protein kinase(AMPK)signaling pathway and mitochondrial energy metabolism in vascular dementia(VD)rats,so as to investigate its potential mechanisms underlying prevention and treatment of VD.Methods Male SD rats were randomly divided into sham surgery group,model group,EA group,non-acupoint group,and EA+inhibitor group,with 8 rats in each group.Except for the sham surgery group,VD model was established in other 4 groups by permanent bilateral common carotid artery ligation.Rats in the EA group received EA at"Baihui"(GV2o)and"Shenting"(GV24),while rats in the non-acupoint group received EA at the non-acupoint 10 mm above the iliac crest bilaterally.The EA frequency was 2 Hz/15 Hz,duration was 30 min per session,once a day,for 7 d continuously.Rats in the EA+inhibitor group received intraperitoneal injection of AMPK inhibitor Compound C 2 h before EA intervention.After intervention,Morris water maze test was used to evaluate the learning and memory ability of rats.ELisA was used to detect the contents of mitochondrial complexes I-IV in the hippocampus.Colorimetric method was used to detect the content of adenosine triphosphate(ATP)in the hippocampus.Transmission electron microscopy was used to observe the ultrastructure of hippocampal mitochondria.Western blot was used to detect the protein expressions of hippocampal AMPKα1,fatty acid translocase(FAT)/cluster of differentiation 36(CD36),and acetyl-CoA carboxylase 2(Acc2).Results Compared with the sham surgery group,the escape latency and total distance traveled of rats in the model group increased(P<0.01),while the time spent in the target quadrant and the number of original platform crossings decreased(P<0.01).The contents of mitochondrial complexes I-IV and ATP in the hippocampus decreased(P<0.01),mitochondrial structure was severely damaged,and the expressions of hippocampal AMPKα1,FAT/CD36 proteins decreased(P<0.01),while ACC2 expression increased(P<0.01).Compared wi

关 键 词：血管性痴呆 电针 线粒体 能量代谢 腺苷酸活化蛋白激酶 

分 类 号：R245.97[医药卫生—针灸推拿学]