电针对阿尔茨海默病小鼠海马髓鞘相关蛋白表达的影响  

Effects of electroacupuncture on the expression of myelin-related proteins in the hippocampus of mice with Alzheimer's disease

作　　者：李金全 董卫国[2] 温若兰 郭婉清 温辰光 黄俊燕 杨少敏 林岚[2] 刘玲珑 LI Jin-quan;DONG Wei-guo;WEN Ruo-lan;GUO Wan-qing;WEN Chen-guang;HUANG Jun-yan;YANG Shao-min;LIN Lan;LIU Ling-long(College of Acupuncture and Moxibustion,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China;College of Integrated Chinese and Western Medicine,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China;The Third People's Hospital of Fujian Province,Fuzhou 350108)

机构地区：[1]福建中医药大学针灸学院,福州350122 [2]福建中医药大学中西医结合学院,福州350122 [3]福建中医药大学附属第三人民医院,福州350108

出　　处：《针刺研究》2025年第1期57-64,共8页Acupuncture Research

基　　金：国家自然科学基金项目(No.81973923);福建省自然科学基金面上项目(No.2022J01348);福建省卫健委科技计划项目(No.2021zyjc23)。

摘　　要：目的:观察电针对快速老化模型SAMP8小鼠焦虑样行为、学习记忆能力及海马髓鞘相关蛋白表达的影响,从而探讨电针改善阿尔茨海默病(AD)的作用机制。方法:将24只7月龄雄性SAMP8小鼠随机分成模型组和电针组,每组12只;以12只同月龄雄性正常老化SAMR1小鼠作为对照组。电针组选取“百会”“大椎”“肾俞”,每日电针20 min,每8 d为1个疗程,每个疗程间隔2 d,共计3个疗程。以旷场实验观察小鼠焦虑样行为,Morris水迷宫实验观察小鼠学习记忆能力,LFB染色法观察小鼠脑组织海马区髓鞘脱失情况,免疫荧光染色法检测海马CA1区髓鞘碱性蛋白(MBP)、髓鞘蛋白脂质蛋白(PLP)的阳性表达水平,实时荧光定量PCR法检测海马组织MBP、硫酸软骨素蛋白聚糖(NG2)和PLP的mRNA表达水平,Western blot法检测海马组织MBP、NG2、PLP、髓鞘相关糖蛋白(MAG)、髓鞘少突胶质细胞糖蛋白(MOG)等髓鞘相关蛋白的表达水平。结果:与对照组比较,模型组小鼠旷场实验中央区域活动路程与活动时间均显著缩短(P<0.01),Morris水迷宫实验逃避潜伏期延长(P<0.01),穿越原平台次数及原平台象限停留时间减少(P<0.01),海马区LFB染色较浅、髓鞘纤维排列稀疏,海马CA1区MBP、PLP的阳性表达,海马组织MBP、NG2、PLP mRNA表达水平,海马组织NG2、MAG、MOG、PLP、MBP蛋白表达水平均明显降低(P<0.01);与模型组比较,电针组小鼠旷场实验中央区域活动路程与活动时间均显著延长(P<0.01),Morris水迷宫实验逃避潜伏期缩短(P<0.01)、穿越原平台次数及原平台象限停留时间增加(P<0.01),海马区LFB染色加深、髓鞘纤维排列较为致密,海马CA1区MBP、PLP的阳性表达水平,海马组织MBP、NG2、PLP mRNA表达水平,海马组织NG2、MAG、MOG、PLP、MBP蛋白表达水平升高(P<0.01,P<0.05)。结论:电针可改善SAMP8小鼠焦虑样行为与学习记忆能力,其机制可能与上调海马区髓鞘相关蛋白表达,进而�Objective To observe the effects of electroacupuncture(EA)onanxiety-likebehavior,learning and memory ability and expressions of myelin-related proteins in the hippocampus of senescence accelerated mice prone 8(SAMP8),so as to explore the mechanism of EA in improving Alzheimer's disease(AD)from the perspective of myelin sheath injury and regeneration.Methods Twenty-four male SAMP8mice were randomly and equally divided into model group and EA group.Twelve male SAMR1 mice of the same age as SAMP8 mice were used as the control group.The rats in the EA group received acupuncture at"Baihui"(GV20),"Dazhui"(GV14)and"Shenshu"(BL23),among which GV14 and BL23 received EA intervention(1.5-2 mA,2 Hz),the intervention lasted for 20 min once daily for a total of 3 courses,with every 8 d as a course and an interval of 2 d between each 2 courses.The anxiety-like behavior of mice was observed by open field test.The learning and memory ability of mice was observed by Morris water maze test.The neuronal demyelination in the hippocampus of mice was observed after luxol fast blue(LFB)staining.The positive expressions of myelin basic protein(MBP)and myelin protein lipid protein(PLP)in hippocampal CA1 region were detected by immunofluorescence.The mRNA expression levels of MBP,chondroitin sulfate proteoglycan(NG2)and PLP,and the protein expression levels of myelin-associated proteins MBP,NG2,PLP,myelin-associated glycoprotein(MAG)and myelin oligodendrocyte glycoprotein(MOG)in the hippocampus were detected by qPCR and Western blot,respevtively.Results Compared with the control group,the activity distance and activity time in the central region in open field test of the model group were significantly shortened(P<0.01),the escape latency of the Morris water maze test was prolonged(P<0.01),the number of crossing the original platform and the residence time in the original platform quadrant were decreased(P<0.01).The LFB staining in the hippocampus was shallow,the myelin fibers were sparse,the positive expressions of MBP and PLP in the hippo

关 键 词：阿尔茨海默病 电针 髓鞘 学习记忆能力 SAMP8小鼠 

分 类 号：R245.97[医药卫生—针灸推拿学]