电针对衰老小鼠肾脏老化及肾脏磷脂酰肌醇3-激酶/蛋白激酶B信号通路的影响  

Effect of electroacupuncture on renal aging and renal phosphatidylinositide 3-kinase/protein kinase B signaling in aging mice

作　　者：陈江敏 杜艳军 田青[3] 王丽 刘欣媛 李蔚娴 许淑鱼 CHEN Jiang-min;DU Yan-jun;TIAN Qing;WANG Li;LIU Xin-yuan;LI Wei-xian;XU Shu-yu(College of Acupuncture-moxibustion and Orthopaedics,Hubei University of Chinese Medicine,Wuhan 430061,China;Hubei Shizhen Laboratory,Wuhan 430061;Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030)

机构地区：[1]湖北中医药大学针灸骨伤学院,武汉430061 [2]湖北时珍实验室,武汉430061 [3]华中科技大学同济医学院,武汉430030

出　　处：《针刺研究》2025年第1期84-89,共6页Acupuncture Research

基　　金：国家自然科学基金项目(No.82374564、82074566);国家中医药管理局“全国名中医传承工作室”建设项目(No.国中医药人教发[2022]5号);湖北省联合基金重点项目(No.2023AFD112)。

摘　　要：目的:观察电针对衰老小鼠老化肾脏病理形态及磷脂酰肌醇3-激酶/蛋白激酶B(PI3K/Akt)信号通路的影响,探讨电针延缓肾脏老化的可能机制。方法:7月龄雄性快速老化SAMP8小鼠随机分为模型组和电针组,每组10只;10只同月龄雄性抗快速老化SAMR1小鼠为正常组。电针组针刺“百会”、电针双侧“肾俞”,每次15 min,1次/d,10 d为1个疗程,疗程间间隔1 d,共4个疗程。观察各组小鼠一般情况;HE染色法观察肾脏病理形态改变,Masson染色评估肾脏纤维化程度,Western blot法检测肾组织肌成纤维标志物α-平滑肌肌动蛋白(α-SMA)、白细胞介素(IL)-1β、IL-4和PI3K、Akt蛋白表达水平。结果:与正常组比较,模型组小鼠毛发光泽度明显下降,日常活动、进食饮水量及对外界刺激反应均欠佳;肾小球萎缩、硬化,肾小管肥大、排列紊乱,炎性细胞浸润明显,肾间质胶原纤维明显增多;肾组织α-SMA、IL-1β、PI3K、Akt蛋白表达水平升高(P<0.001,P<0.05, P<0.01),IL-4蛋白表达水平降低(P<0.01)。与模型组比较,电针组小鼠皮毛尚有光泽,日常活动、进食饮水量及对外界刺激反应均尚可;肾小球和肾小管病理形态均明显改善,肾间质胶原纤维明显减少;肾组织α-SMA、IL-1β、PI3K、Akt蛋白表达水平降低(P<0.05),IL-4表达水平升高(P<0.05)。结论:电针可通过抑制IL-1β、α-SMA在肾脏的过度表达和增加IL-4表达改善SAMP8小鼠肾脏纤维化,进而延缓肾老化,抑制PI3K/Akt炎性通路可能是电针抗肾脏纤维化的可能机制之一。Objective To observe the effect of electroacupuncture(EA)at"Baihui"(Gv20)and"Shenshu"(BL23)on the morphological changes of aging kidney and phosphatidylinositol 3-kinase/protein kinases B(PI3K/Akt)signaling in SAMP8 mice,so as to explore its mechanisms of delaying renal aging.Methods A total of 20 seven-month-old male SAMP8 mice were randomly divided into model group and EA group,with 10 mice in each group.The normal group consisted of 10 male SAMR1 mice with the same age.In the EA group,acupuncture was performed at GV20 and bilateral BL23 respectively.EA(2 Hz,1 mA)was applied to bilateral BL23 for 15 min every time,once a day,10 days as a course for a total of 4 courses,the interval between courses was one day.The general condition of the mice in each group was observed during the intervention period.After the intervention,H.E.staining was used to observe the morphological changes of aging kidney.Masson staining was used to evaluate the degree of renal fibrosis.The relative protein expression levels of alpha smooth musle action(α-SMA),interleukin-1β(IL-1β),interleukin-4(IL-4),Pl3K and Akt in renal tissue were detected by Western blot.Results Compared with thenormal group,mice inthe model group showed dull hair,reduced daily activity and food and water intake,and poor response to external stimulation,and the relative protein expression levels ofα-SMA,IL-1β,PI3K and Akt in renal tissue were significantly increased(P<0.001,P<0.05,P<0.01),while the protein expression level of IL-4 was significantly decreased(P<0.01).After EA intervention,compared with the model group,the general condition of rats in the EA group was significantly improved,and the relative protein expression levels ofα-SMA,IL-1β,PI3K and Akt were obviously decreased(P<0.05),while the level of IL-4 was significantly increased(P<0.05).H.E.staining and Masson staining showed that the glomeruli of the mice in the model group were atrophied and sclerotic,the renal tubules were hypertrophied and disorganized,the inflammatory cell infiltration was ob

关 键 词：电针 衰老 磷脂酰肌3激酶/蛋白激酶B信号通路 肾纤维化 

分 类 号：R245.97[医药卫生—针灸推拿学]