工具病毒载体在针刺镇痛相关脑环路机制研究中的应用  

Application of Tool Virus Vector in Acupuncture Analgesia Research

作　　者：马翠 叶钰娟 严兴科 Ma Cui;Ye Yu-juan;Yan Xing-ke(College of Acupuncture and Massage,Gansu University of Traditional Chinese Medicine,Lanzhou 730101,China)

机构地区：[1]甘肃中医药大学针灸推拿学院,甘肃兰州730000

出　　处：《针刺研究》2025年第1期116-122,共7页Acupuncture Research

基　　金：2023年度甘肃省省级重点人才项目(No.2023-20);2021年甘肃省科技计划项目(No.21JR1RA266);国家自然科学基金项目(No.81460744)。

摘　　要：针刺镇痛临床疗效确切,但其中枢脑环路机制尚未完全阐明,工具病毒载体技术为针刺镇痛中枢环路机制研究提供了有效手段。本文简述了工具病毒载体的分类及特性,以及近年来工具病毒载体及其携载的功能元件光/化学遗传学技术在疼痛及针刺镇痛研究中的应用概况。目前,在针刺镇痛中枢机制研究中,工具病毒结合化学遗传学技术主要用于研究前喙扣带皮层→腹外侧中脑导水管周围灰质(rACC→vlPAG)、前扣带回皮层→丘脑(rACC→Th)及腹外侧中脑导水管周围灰质→海马(vlPAG→HPC)等神经环路机制;工具病毒结合光遗传学技术主要用于研究内侧前额边缘下皮层→伏隔核(IL→NAc)等神经环路。工具病毒及光/化学遗传学技术既可靶向性、精确性地标记特定神经元或神经环路,又能动态、实时监测神经网络功能,为针刺镇痛脑环路机制研究带来了新的实验手段和突破口。IThe clinical efficacy of acupuncture analgesia is definite,but its mechanisms of the central nervous system have not yet been fully elucidated.The virus vector technique provides an effective means for the study of the central nervous system mechanism of acupuncture analgesia.We,in this article,briefly introduced the classification and characteristics of virus vectors,reviewed and analyzed their application and their functional components carried by and optical/chemical genetics technologies they carry in pain and acupuncture analgesia research in recent years.At present,in the research on the central mechanism of acupuncture analgesia,the viral vectors combined with chemical genetics technology is mainly used to explore the neural circuit mechanisms such as rostral anterior cingulate cortex(rACC)→ventrolateral periaqueductal gray(rACC-→vlPAG),rACC-→thalamus(rACC-→Th)and ventrolateral periaqueductal gray-→hippocampus(viPAG-→HPC).The viral vectors combined with optogenetics technology is mainly used to explore the neural circuits from the prefrontal infralimbic cortex to the nucleus accumbens(IL->NAc).The viral vectors and optical/chemical genetics technology can not only target and accurately mark the specific neurons or neural circuits,but also dynamically and real-time monitor the function of neural networks,bringing new experimental means and breakthroughs for the research of acupuncture analgesia mechanism of the brain circuits.

关 键 词：工具病毒 针刺镇痛 光遗传学 化学遗传学 脑环路 

分 类 号：R245[医药卫生—针灸推拿学]