甲基麦冬黄烷酮A改善高糖诱导H9c2心肌细胞损伤的作用研究  

Effects of methyl oxofulnonone A ameliorates hyperglucosee-induced H9c2 cardiomyocyte injury

作　　者：欧阳明慧 郭晓彤 刘婷 程丽颖 牛子长[2] 毛浩萍[1] OUYANG Ming-hui;GUO Xiao-tong;LIU Ting;CHENG Li-ying;NIU Zi-chang;MAO Hao-ping(Institute of Chinese Medicine,Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China;Cardiovascular Department,TheFirst Affiliated Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China)

机构地区：[1]天津中医药大学中医药研究院,天津301617 [2]天津中医药大学第一附属医院心血管科,天津301617

出　　处：《中国临床药理学杂志》2025年第1期45-49,共5页The Chinese Journal of Clinical Pharmacology

基　　金：国家自然科学基金面上基金资助项目(82174180)。

摘　　要：目的研究甲基麦冬黄烷酮A(META)对高糖(HG)诱导H9c2细胞损伤的作用及机制。方法将H9c2细胞分为对照组(正常培养)、模型组(55mmol·L^(-1)葡萄糖)和低、中、高剂量实验组(55mmol·L^(-1)葡萄糖+12.5、25.0、50.0μmol·L^(-1)META)。用细胞计数盒法-8法检测细胞活力,用DCFH-DA荧光探针检测细胞内活性氧(ROS)水平,用荧光素酶检测细胞内腺苷三磷酸(ATP)含量,用蛋白质印迹法检测自噬相关蛋白的表达水平。结果对照组、模型组和中、高剂量实验组的72h细胞光密度值分别为0.91±0.03、0.61±0.01、0.69±0.02和0.72±0.03,ROS水平分别为(40.75±1.53)%、(43.73±1.30)%、(30.87±1.27)%和(28.28±1.43)%,ATP含量分别为(8.16±0.71)、(4.03±0.29)、(5.29±0.31)和(5.83±0.31)nmol·mg^(-1),自噬相关基因5蛋白相对表达水平分别为1.05±0.06、1.46±0.09、0.98±0.11和0.89±0.09,泛素结合蛋白P62蛋白相对表达水平分别为1.05±0.10、0.55±0.13、0.89±0.04和0.98±0.04,微管相关蛋白1轻链3Ⅱ/Ⅰ蛋白比值分别为1.09±0.09、1.82±0.05、1.67±0.29和1.09±0.15。中、高剂量实验组和对照组的上述指标与模型组相比,在统计学上差异均有统计学意义(P<0.05,P<0.01)。结论META显著改善高糖导致的H9c2心肌细胞损伤,改善氧化应激状态,保护线粒体呼吸功能和抑制细胞自噬。Objective To investigate the effect and mechanism of methyl oxofulnonone A(META)on high glucose(HG)-induced H9c2 cell injury.Methods H9c2 cells were divided into control group(normal culture),model group(55 mmol·L^(-1)gglucose)and experimental-L,-M,-H groups(55 mmol·L^(-1)glucose+12.5,25.0,50.0μmol·L^(-1) META).Cell viability was detected by cell counting kit-8;intracellular reactive oxygen species(ROS)level was detected by DCFH-DA fluorescent probe;intracellular adenosine triphosphate(ATP)content was detected by luciferase;and autophagy-related protein expression was detected by Western blotting.Results The optical density values of 72-hour cells in the control group,model group and experimental-M,-H groups were 0.91±0.03,0.61±0.01,0.69±0.02 and 0.72±0.03;the ROS levels were(40.75±1.53)%,(43.73±1.30)%,(30.87±1.27)% and(28.28±1.43)%;the ATP contents were(8.16±0.71),(4.03±0.29),(5.29±0.31)and(5.83±0.31)nmol·mg^(-1);the relative expression levels of autophagy-related gene 5 protein were 1.05±0.06,1.46±0.09,0.98±0.11 and 0.89±0.09;the relative expression levels of ubiquitin-binding protein were 1.05±0.10,0.55±0.13,0.89±0.04 and 0.98±0.04;the ratios of microtubule-associated protein 1 light chain 3Ⅱ/Ⅰ protein were 1.09±0.09,1.82±0.05,1.67±0.29 and 1.09±0.15,respectively.Among the above indicators,there were statistically significant differences between the model group and the control and experimental-M,-H groups(P<0.05,P<0.01).Conclusion META significantly ameliorates H9c2 cardiomyocyte damage caused by high glucose,ameliorates oxidative stress,protects mitochondrial respiration and inhibits autophagy.

关 键 词：甲基麦冬黄烷酮A 高糖 H9C2心肌细胞 自噬 心肌损伤 

分 类 号：R28[医药卫生—中药学]