一测多评法同时测定通宣理肺丸中9种成分的含量  

Simultaneous content determination of nine constituents in Tongxuan Lifei Pills by QAMS

作　　者：刘靖 靳婉君 郭朝晖 倪琳 LIU Jing;JIN Wan-jun;GUO Zhao-hui;NI Lin(College of Pharmacy,Gansu University of Traditional Chinese Medicine,Lanzhou 730000,China;State Key Laboratory for Quality Control of Chinese Medicinal Materials and Decoction Pieces,Gansu Provincial Engineering Laboratory of Chinese and Tibetan Medicine Inspection and Testing Technology,Gansu Provincial Institute for Drug Control,Lanzhou 730030,China)

机构地区：[1]甘肃中医药大学药学院,甘肃兰州730000 [2]甘肃省药品检验研究院,国家中药材及饮片质量控制重点实验室,甘肃省中藏药检验检测技术工程实验室,甘肃兰州730030

出　　处：《中成药》2025年第1期7-13,共7页Chinese Traditional Patent Medicine

基　　金：甘肃省药品科研项目(2022GSMPA-KL12,2022GSMPA-KL14)。

摘　　要：目的建立一测多评法同时测定通宣理肺丸中黄芩素、黄芩苷、橙皮苷、柚皮苷、新橙皮苷、迷迭香酸、甘草苷、甘草酸铵、白花前胡甲素的含量。方法分析采用SVEA C 18色谱柱(250 mm×4.6 mm,5μm);流动相乙腈-水(含0.1%磷酸),梯度洗脱;体积流量1.0 mL/min;柱温30℃;检测波长250 nm。以黄芩苷为内标,计算其他8种成分的相对校正因子,测定其含量。结果9种成分在各自范围内线性关系良好(r≥0.9991),平均加样回收率96.15%~101.58%,RSD 0.75%~1.74%。一测多评法所得结果与外标法接近。结论该方法准确稳定,重复性好,可用于通宣理肺丸的质量控制。AIM To establish a quantitative analysis of multi-components by single-marker(QAMS)method for the simultaneous content determination of baicalin,baicalin,hesperidin,naringin,neohesperidin,rosinic acid,glycyrrhizin,ammonium glycyrrhizate and prehumetin in Tongxuan Lifei Pills.METHODS The analysis was performed on a 30℃thermostatic SVEA C 18 column(4.6 mm×250 mm,5μm),with the mobile phase comprising of acetonitrile-water(containing 0.1%phosphoric acid)flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 250 nm.Baicalin was used as an internal standard to calculate the relative correction factors of the other eight constituents,after which the content determination was made.RESULTS Nine constituents showed good linear relationships within their own ranges(r≥0.9991),whose average recoveries were 96.15%-101.58%with the RSDs of 0.75%-1.74%.The result obtained by QAMS approximated those obtained by external standard method.CONCLUSION This accurate,stable and reproducible method can be used for the quality control of Tongxuan Lifei Pills.

关 键 词：通宣理肺丸 化学成分 含量测定 一测多评 

分 类 号：R927.2[医药卫生—药学]