基于HPLC指纹图谱结合化学计量学评价“桑蒂”质量  

Quality evaluation of“Sangdi”based on HPLC fingerprints combined with chemometrics

作　　者：刘平 罗世英 李梦佳 谭小燕 孙建彬[3] 罗维早[3] 唐策[2] 张艺[2] LIU Ping;LUO Shi-ying;LI Meng-jia;TAN Xiao-yan;SUN Jian-bin;LUO Wei-zao;TANG Ce;ZHANG Yi(School of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;School of Ethnic Medicine,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;Chongqing Municipal Academy of Chinese Materia Medica,Chongqing 400060,China)

机构地区：[1]成都中医药大学药学院,四川成都611137 [2]成都中医药大学民族医药学院,四川成都611137 [3]重庆市中药研究院,重庆400060

出　　处：《中成药》2025年第1期14-21,共8页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金面上项目(81973573);国家重点研发计划项目(2023YFC3504400)。

摘　　要：目的基于HPLC指纹图谱结合化学计量学评价藏药“桑蒂”质量。方法分析采用Welch Ultimate AQ-C 18色谱柱(250 mm×4.6 mm,5μm);流动相乙腈-0.2%磷酸,梯度洗脱;体积流量1 mL/min;柱温30℃;检测波长245 nm,进行聚类分析、主成分分析和正交偏最小二乘法判别分析,测定龙胆苦苷、獐牙菜苷、芒果苷、异荭草苷、8-羟基-1,3,5-三甲氧基口山酮(R2)、1,8-二羟基-3,7-二甲氧基口山酮(R3)含量。结果15批样品指纹图谱中有18个共有峰,相似度均大于0.90。6种成分在各自范围内线性关系良好(R 2≥0.9992),平均加样回收率96.93%~103.58%,RSD 0.82%~2.9%。各批样品聚为2类,4个主成分累积方差贡献率为86.404%,筛选出芒果苷、龙胆苦苷、异荭草苷为质量差异标志物。结论该方法稳定可靠,重复性好,可为全面评价“桑蒂”质量提供参考。AIM To evaluate the quality of Tibetan medicine“Sangdi”based on HPLC fingerprints combined with chemometrics.METHODS The analysis was performed on a 30℃thermostatic Welch Ultimate AQ-C 18 column(250 mm×4.6 mm,5μm),with the mobile phase comprising of acetonitrile-0.2%phosphoric acid flowing at 1 mL/min in a gradient elution manner,and the detection wavelength was set at 245 nm,after which cluster analysis,principal component analysis and orthogonal partial least squares discriminant analysis were performed,the contents of gentiopicroside,sweroside,mangiferin,isoorientin,8-hydroxy-1,3,5-trimethoxyxanthone(R2)and 1,8-dihydroxy-3,7-dimethoxyxanthone(R3)were determined.RESULTS There were 18 common peaks in the fingerprints for 15 batches of samples with the similarities of more than 0.90.Six constituents showed good linear relationships within their own ranges(R 2≥0.9992),whose average recoveries were 96.93%-103.58%with the RSDs of 0.82%-2.9%.Various batches of samples were clustered into 2 categories,4 principal components demonstrated the accumulative variance contribution rate of 86.404%,mangiferin,gentiopicroside and isoorientin were taken as quality difference markers.CONCLUSION This stable,reliable and reproducibe method can provide a reference for the comprehensive quality evaluation of“Sangdi”.

关 键 词：“桑蒂” 质量评价 HPLC指纹图谱 聚类分析 主成分分析 正交偏最小二乘法判别分析 化学成分 含量测定 

分 类 号：R282[医药卫生—中药学]