降脂理肝汤提取工艺优化及其对HepG2细胞脂质蓄积的改善作用  

Optimization of extraction process for Jiangzhi Ligan Decoction and its effects on improving lipid deposition in HepG2 cells

作　　者：季敬 孙妍华 侯付强 姜昊 何珊 JI Jing;SUN Yan-hua;HOU Fu-qiang;JIANG Hao;HE Shan(Shandong University of Traditional Chinese Medicine,Jinan 250355,China)

机构地区：[1]山东中医药大学,山东济南250355

出　　处：《中成药》2025年第1期36-42,共7页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金青年科学基金项目(81903944);国家中医药管理局高水平中医药重点学科建设项目(zyyzdxk-2023124);山东省中医药重点学科建设项目(鲁卫中医药科教字[2022]4号)。

摘　　要：目的优化降脂理肝汤提取工艺,并考察其对HepG2细胞脂质蓄积的改善作用。方法在单因素试验基础上,以提取时间、提取次数、溶剂用量为影响因素,丹参素、荷叶碱、儿茶素含量及浸膏得率的综合评分为评价指标,Box-Behnken响应面法优化提取工艺。采用棕榈酸诱导的HepG2细胞建立非酒精性脂肪肝模型,考察含药血清对TC、TG水平的影响。结果最佳条件为提取时间90 min,提取次数3次,溶剂用量11倍,综合评分为99.34分。与对照组比较,模型组TC、TG水平升高(P<0.01);与模型组比较,降脂理肝汤高剂量组TC、TG水平降低(P<0.05,P<0.01),以优化后更明显(P<0.05,P<0.01)。结论降脂理肝汤可减轻棕榈酸诱导的HepG2细胞脂质蓄积,其提取工艺优化后的药效优于文献报道。AIM To optimize the extraction process for Jiangzhi Ligan Decoction,and to investigate its effects on improving lipid deposition in HepG2 cells.METHODS With extraction time,extraction frequency and solvent consumption as influencing factors,comprehensive score for tanshensu,lotine,catechin contents and extract yield as an evaluation index,the extraction process was optimized by Box-Behnken response surface method on the basis of single factor test.Palmitic acid-induced HepG2 cells were adopted in the establishment of non-alcoholic fatty liver disease model,after which the effect of drug-containing serum on TC and TG levels was investigated.RESULTS The optimal conditions were determined to be 90 min for extraction time,three times for extraction frequency,and 11 times for solvent consumption.Compared with the control group,the model group demonstrated increased TG,TC levels(P<0.01);compared with the model group,the Jiangzhi Ligan Decoction high-dose group displayed decreased TG,TC levels(P<0.05,P<0.01),especially for those after optimization(P<0.05,P<0.01).CONCLUSION Jiangzhi Ligan Decoction can alleviate lipid deposition in HepG2 cells induced by palmitic acid,whose efficacy after extraction process optimization is better than that reported in literature.

关 键 词：降脂理肝汤 提取工艺 Box-Behnken响应面法 HEPG2细胞 脂质蓄积 非酒精性脂肪肝 

分 类 号：R284.2[医药卫生—中药学]