白背叶楤木芽苞化学成分及其体外抗炎活性研究  

Chemical constituents from the buds of Aralia chinensis var.nuda and their in vitro anti-inflammatory activities

作　　者：王娟 袁圆 尹芃程 李绍花 陈帅 钱海珊 李红芳 何红平 李宝晶 WANG Juan;YUAN Yuan;YIN Peng-cheng;LI Shao-hua;CHEN Shuai;QIAN Hai-shan;LI Hong-fang;HE Hong-ping;LI Bao-jing(College of Traditional Chinese Medicine,Yunnan University of Chinese Medicine,Kunming 650500,China;Yuxi Agriculture Vocation-Technical College,Yuxi 653100,China;The First Affiliated Hospital of Yunnan University of Chinese Medicine,Kunming 650500,China;Yunnan Key Laboratory of Southern Medicinal Utilization,Yunnan University of Chinese Medicine,Kunming 650500,China)

机构地区：[1]云南中医药大学中药学院,云南昆明650500 [2]玉溪农业职业技术学院,云南玉溪653100 [3]云南中医药大学第一附属医院,云南昆明650500 [4]云南中医药大学,云南省南药可持续利用研究重点实验室,云南昆明650500

出　　处：《中成药》2025年第1期101-107,共7页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金项目(81860760,82260832);云南省中医联合专项(202001AZ070001-044,202101AZ070001-159);云南省重大科技专项(202002AA100007)。

摘　　要：目的研究白背叶楤木Aralia chinensis L.var.nuda Nakai芽苞化学成分及其体外抗炎活性。方法白背叶楤木芽苞70%乙醇提取物采用硅胶、Sephadex LH-20、ODS、半制备HPLC进行分离纯化,根据理化性质及波谱数据鉴定化合物结构。采用RAW264.7模型评价其体外抗炎活性。结果从中分离得到16个化合物,分别鉴定为4-(2,2-dibutoxyethyl)phenol(1)、trans-linalool-3,7-oxide-6-O-β-D-glucopyranoside(2)、2′-O-(9Z,12Z,15Z-octadecatrienoyl)glycerylβ-D-galactopyranoside(3)、quercetin-3-O-β-D-glucopyranoside(3′→O-3)quercetin-3-O-β-D-galactopyranoside(4)、syringaresinol-4′-O-β-D-glucopyranoside(5)、p-hydroxybenzaldehyde(6)、7α-hydroxystigmasterol 3-O-β-D-glucopyranoside(7)、trans-p-hydroxycinnamic acid methyl ester(8)、funingensin A(9)、3,4-dihydroxy-acetophenone(10)、N-acetyltyramine(11)、3,4-di-O-caffeoyl quinic acid(12)、chlorogenic acid(13)、aralia cerebroside(14)、caffeic acid methyl ester(15)、tetradecanoic acid(16)。化合物8、10、12、13的IC 50值分别为(22.19±1.59)、(35.25±1.30)、(13.38±0.72)、(15.73±1.16)μmol/L。结论化合物1为新化合物,2~13为首次从楤木属植物中分离得到。化合物8、10、12、13具有良好的体外抗炎活性。AIM To study the chemical constituents from the buds of Aralia chinensis L.var.nuda Nakai and their in vitro anti-inflammatory activities.METHODS The 70%ethanol extract from the buds of A.chinensis var.nuda was isolated and purified by silica gel,Sephadex LH-20,ODS and semi-preparative HPLC,then the structures of compounds were identified by physicochemical properties and spectral data.Their anti-inflammatory activities in vitro were evaluated by RAW264.7 model.RESULTS Sixteen compounds were isolated and identified as 4-(2,2-dibutoxyethyl)phenol(1),trans-linalool-3,7-oxide-6-O-β-D-glucopyranoside(2),2′-O-(9Z,12Z,15Z-octadecatrienoyl)glycerylβ-D-galactopyranoside(3),quercetin-3-O-β-D-glucopyranoside(3′→O-3)quercetin-3-O-β-D-galactopyranoside(4),syringaresinol-4′-O-β-D-glucopyranoside(5),p-hydroxybenzaldehyde(6),7α-hydroxystigmasterol 3-O-β-D-glucopyranoside(7),trans-p-hydroxycinnamic acid methyl ester(8),funingensin A(9),3,4-dihydroxy-acetophenone(10),N-acetyltyramine(11),3,4-di-O-caffeoyl quinic acid(12),chlorogenic acid(13),aralia cerebroside(14),caffeic acid methyl ester(15),tetradecanoic acid(16).The IC 50 values of compounds 8,10,12 and 13 were(22.19±1.59),(35.25±1.30),(13.38±0.72),(15.73±1.16)μmol/L,respectively.CONCLUSION Compound 1 is a new compound,2-13 are isolated from genus Aralia for the first time.Compounds 8,10,12,13 exhibit significant in vitro anti-inflammatory activities.

关 键 词：白背叶楤木 芽苞 化学成分 分离鉴定 体外抗炎活性 

分 类 号：R284.1[医药卫生—中药学]