土家药刺老苞水提物对维甲酸诱导的骨质疏松大鼠股骨CYP17A1、CYP19A1的影响  

Effect of Tujia Medicine Aralia Chinensis L Water Extract on CYP17A1 and CYP19A1 in Rats with Retinoic Acid-induced Osteoporosis

作　　者：刘春艳 牛建均 田永松 胡贵瑶 曾凡勇 王焕珍 张永红 晏朝操 文琴琴 柴艺汇 LIU Chunyan;NIU Jianjun;TIAN Yongsong;HU Guiyao;ZENG Fanyong;WANG Huanzhen;ZHANG Yonghong;YAN Chaocao;WEN Qinqin;CHAI Yihui(Institute of Tujia Medicine,Dejiang County Hospital of Ethnic Traditional Chinese Medicine,Guizhou Province,Dejiang 565200,China;Department of General Surgery,Dejiang County People’s Hospital,Guizhou Province,Dejiang 565200,China;School of Basic Medicine,Guizhou University of Traditional Chinese Medicine,Guizhou Province,Guiyang 550000,China)

机构地区：[1]贵州省德江县民族中医院土家医药研究所,贵州德江565200 [2]贵州省德江县人民医院普外科,贵州德江565200 [3]贵州中医药大学基础医学院,贵州贵阳550000

出　　处：《中国中医药现代远程教育》2025年第4期96-99,共4页Chinese Medicine Modern Distance Education of China

基　　金：贵州省中医药管理局科研项目[No.QZYY-2021-092,QZYY-2023-109];贵州省中医药管理局民族民间医术挖掘整理及活态传承收集项目[No.黔中医药函[2020]101号];贵州中医药大学大学生创新创业训练计划项目[No.贵中医大创合字[2022]43号];贵州中医药大学科研项目[No.2021]。

摘　　要：目的 探讨刺老苞防治骨质疏松症(OP)的分子机制。方法 将36只SD大鼠随机分为空白组,模型组,阳性对照组(维生素D组),刺老苞水提取物高、中、低剂量组,各6只。以苏木素-伊红(HE)染色观察大鼠股骨病理学变化,逆转录-聚合酶链反应(RT-PCR)法检测股骨组织细胞色素P450羟化酶(CYP17A1)、细胞色素P450 19A1(CYP19A1)mRNA表达情况。结果 与空白组比较,模型组大鼠HE染色镜下骨小梁明显变细伴断裂、稀疏,骨小梁数量重度减少、间隙增大。与模型组比较,刺老苞水提取物高剂量组大鼠可见骨小梁数量明显增多、骨小梁局部明显增粗,且刺老苞水提取物高剂量组优于刺老苞水提取物低、中剂量组。与空白组比较,模型组大鼠股骨中CYP17A1、CYP19A1 mRNA相对表达量显著降低(P<0.01);与模型组比较,刺老苞水提取物高剂量组、阳性对照组大鼠股骨中CYP17A1、CYP19A1 mRNA相对表达量显著升高(P<0.01)。结论 刺老苞通过调节股骨组织CYP17A1、CYP19A1 mRNA表达情况,发挥干预OP的作用。Objective To explore the molecular mechanism of Aralia Chinensis L in preventing osteoporosis(OP).Methods A total of 36 SD rats were randomly divided into blank group,model group,positive control group(vitamin D group),high-dose,medium-dose and low-dose groups with 6 rats each.The pathological changes of femur were observed by hematoxylin-eosin(HE)staining,and the mRNA expressions of cytochrome P450 hydroxylase(CYP17A1)and cytochrome P45019A1(CYP19A1)were detected by reverse transcription-polymerase chain reaction(RT-PCR).Results Compared with the blank group,the trabecular thinning with fracture,sparsity,severely reduced trabecular number and increased gap.Compared with the model group,the number of trabecules increased significantly in the increasing dose of the group,and the local thickening of the trabecules increased significantly,while the increased dose of the group was better than the low and medium dose groups.Compared with the blank group,the relative expression of CYP17A1 and CYP19A1 mRNA in the model group(P<0.01).Compared with the model group,the relative expression of CYP17A1 and CYP19A1 mRNA in the group and the positive control group(P<0.01).Conclusion By regulating the expression of CYP17A1 and CYP19A1mRNA in femoral tissue,it plays its role in intervening in OP.

关 键 词：骨质疏松症 刺老苞 CYP17A1 CYP19A1 实验研究 

分 类 号：R29[医药卫生—民族医学]