Stat1抑制Foxp3的表达和Treg产生  

Stat 1 inhibits Foxp 3 expression and Treg production

作　　者：郭帅 张格 陈昊 秦安琪 李文婷 张爱红[4] 郑爱华[4] 田枫 郑全辉 GUO Shuai;ZHANG Ge;CHEN Hao;QIN Anqi;LI Wenting;ZHANG Aihong;ZHENG Aihua;TIAN Feng;ZHENG Quanhui(Basic Medicine College,North China University of Science and Technology,Hebei Key Laboratory of Basic Medicine for Chronic Diseases,Tangshan 063210,China;School of Basic Medicine,North China University of Science and Technology,Tangshan 063210,China;Experimental Animal Science Department,Peking University School of Medicine,Beijing 100083,China;Tangshan Workers'Hospital,Tangshan 063000,China)

机构地区：[1]华北理工大学基础医学院河北省慢性疾病基础医学重点实验室,唐山063210 [2]华北理工大学基础医学院,唐山063210 [3]北京大学医学部实验动物科学部,北京100083 [4]唐山市工人医院,唐山063000

出　　处：《中国免疫学杂志》2025年第2期271-275,共5页Chinese Journal of Immunology

基　　金：国家自然科学基金面上项目(81373111);河北省卫生健康委资助项目(20190105)。

摘　　要：目的:探究Stat1对Foxp3的表达和Treg产生的影响。方法:C57BL/6小鼠分为正常对照组和Stat1特异抑制剂氟达拉滨(Flud)处理组。采用流式细胞术检测小鼠脾脏、淋巴结和外周血中CD4~+Foxp3~+Treg比例及Foxp3的表达变化。构建人源性Stat1过表达质粒,转染人乳腺癌MCF-7细胞系,采用RT-qPCR和Western blot检测Foxp3的表达变化。结果:与正常对照组相比,Flud处理组小鼠淋巴结和外周血Treg比例及Foxp3表达增加,而过表达Stat1则导致MCF-7细胞中Foxp3 mRNA及蛋白表达水平显著降低。结论:Stat1抑制Foxp3的表达和Treg的产生。Objective:To explore the influence of Stat1 on Foxp3 expression and production of Treg.Methods:C57BL/6 mice were used and separated into normal control group and Stat1 specific inhibitor Fludarabine(Flud)treatment group.Ratio of CD4+Foxp3+Treg and expression of Foxp3 in spleen,lymph nodes and peripheral blood of mice in each group were detected by flow cytometry.Human Stat1 overexpression plasmid was constructed and transfected into human breast cancer MCF-7 cells,and expression changes of Foxp3 was detected by RT-qPCR and Western blot.Results:Compared with mice in normal control group,proportion of Treg and expression of Foxp3 in lymph nodes and peripheral blood of mice in Flud treatment group were increased,while Stat1 overex‐pression resulted in decreased Foxp3 mRNA and protein expression in MCF-7 cells.Conclusion:Stat1 inhibits expression of Foxp3 and production of Tregs.

关 键 词：STAT1 FOXP3 调节性T细胞 人乳腺癌细胞 MCF-7 

分 类 号：R392.3[医药卫生—免疫学]