肺泡巨噬细胞对屋尘螨诱导的过敏性气道炎症的影响  

Effects of alveolar macrophages on allergic airway inflammation induced by house dust mites

作　　者：程百合 杨文婷 侯飞 唐丽 CHENG Baihe;YANG Wenting;HOU Fei;TANG Li(School of Basic Medicine,Qingdao University,Qingdao 266071,China;State Key Laboratory of Medical Proteomics,Beijing Proteome Research Center,National Center for Protein Sciences(Beijing),Beijing Institute of Lifeomics,Beijing 102206,China)

机构地区：[1]青岛大学基础医学院,青岛266071 [2]军事科学院军事医学研究院生命组学研究所,蛋白质组学国家重点实验室,北京102206

出　　处：《中国免疫学杂志》2025年第2期276-280,286,共6页Chinese Journal of Immunology

基　　金：国家自然科学基金青年基金(31900632)。

摘　　要：目的:研究屋尘螨(HDM)诱导的过敏性气道炎症小鼠模型中免疫细胞的变化,并对肺泡巨噬细胞(AM)在其中的效应功能进行初步探索。方法:气管注射HDM诱导过敏性气道炎症,并在建模前通过气管注射氯膦酸盐脂质体(CL)特异性地对AM进行清除;心脏灌注分离小鼠全肺免疫细胞;气管灌洗分离小鼠肺泡灌洗液免疫细胞;ELISA检测肺泡灌洗液Ⅱ型细胞因子的水平;流式细胞术检测肺脏或肺泡灌洗液免疫细胞的变化;HE染色、PAS染色评价HDM建模后肺脏炎症粒细胞浸润、杯状细胞增生以及黏液分泌情况。结果:在HDM诱导的过敏性气道炎症中,Ⅱ型免疫反应的效应细胞如Ⅱ型先天性淋巴细胞(ILC2)、嗜酸性粒细胞显著增加,组织学分析表明肺组织有大量炎症细胞浸润,杯状细胞增生,黏液分泌增加。而清除AM减缓了HDM诱导的Ⅱ型免疫反应,但促进了中性粒细胞的浸润,加重了肺脏的病理损伤。结论:AM在HDM诱导过敏性气道炎症中发挥重要作用。Objective:To study the changes of immune cells in allergic airway inflammation induced by house dust mites(HDM)in the mouse model,and investigate the effect of alveolar macrophages(AM)on allergic airway inflammation.Methods:Allergic airway inflammation was induced by intratracheal injection of HDM,and AM were specifically depleted by intratracheal injection of chlorophosphonate liposomes(CL)before HDM treatment;the immune cells of mouse lung were isolated by heart perfusion.The immune cells of mouse alveolar lavage fluid were isolated by tracheal lavage.The level of typeⅡcytokines in alveolar lavage fluid was determined by ELISA.The changes of immune cells in mouse lung tissue and alveolar lavage fluid were detected by flow cytometry;HE staining and PAS staining were performed to observe the infiltration of inflammatory cell,goblet cell proliferation and mucus secretion in the lung sections after HDM treatment.Results:The effector cells of typeⅡimmune response,such as group 2 innate lymphoid cells(ILC2)and eosinophils,were significantly increased in allergic airway inflammation induced by HDM in mice.Histological analysis also revealed substantial inflammatory cell infiltration,goblet cells proliferation and mucus secretion in lung tissue.The depletion of AM alleviated HDM induced typeⅡimmune response,but promoted the infiltration of neutrophils and aggravated the pathological injury of the lung.Conclusion:AM plays an important role in HDM induced allergic airway inflammation.

关 键 词：屋尘螨 过敏性气道炎症 肺泡巨噬细胞 嗜酸性粒细胞 Ⅱ型先天性淋巴细胞 

分 类 号：R392.8[医药卫生—免疫学]