紫榆膏对创面愈合大鼠肉芽组织TGF-β/Smad信号通路的影响  

Effect of Ziyu Ointment on TGF-β/Smad signal pathway in granulation tissue of wound healing rats

作　　者：王霞 刘志青 秦志娟 靳艳霞 贾子娟 郭笋 高媛媛[3] 李灵 WANG Xia;LIU Zhiqing;QIN Zhijuan;JIN Yanxia;JIA Zijuan;GUO Sun;GAO Yuanyuan;LI Ling(Department of Obstetrics,Hebei Hospital of Traditional Chinese Medicine,Shijiazhuang 050013,China;Outpatient Department of Hebei Hospital of Traditional Chinese Medicine,Shijiazhuang 050013,China;Delivery Room,Hebei Hospital of Traditional Chinese Medicine,Shijiazhuang 050013,China)

机构地区：[1]河北省中医院产科,石家庄050013 [2]河北省中医院门诊部,石家庄050013 [3]河北省中医院产房,石家庄050013

出　　处：《中国免疫学杂志》2025年第2期387-392,共6页Chinese Journal of Immunology

基　　金：河北省中医药管理局科研计划项目(2020121)。

摘　　要：目的:通过构建背部创伤大鼠模型,探究紫榆膏对大鼠创面愈合过程中肉芽组织和TGF-β及下游Smad蛋白的影响。方法:构建背部创伤大鼠模型,将100只雄性SD大鼠随机分为健康组、背部创伤组、紫榆膏处理组、凡士林处理组、紫榆膏+TGF-β1激活组,每组20只。进行创面形成手术,记录各组大鼠术后5 d、10 d、15 d创面愈合情况;检测各组大鼠血清中IL-6、IL-1β、TNF-α水平;选取15 d创面愈合形成的肉芽组织进行HE、Masson染色,分析计算各组大鼠肉芽组织血管面积占比和胶原蛋白面积占比;荧光定量PCR法检测各组大鼠肉芽组织中TGF-β1、Smad3 mRNA表达水平;Western blot法检测各组大鼠肉芽组织中TGF-β1、Smad3、p-Smad3蛋白表达水平。结果:健康组大鼠具有完整的表皮组织;与健康组相比,背部创伤组大鼠血清IL-6、IL-1β、TNF-α含量、创面肉芽组织TGF-β1 mRNA和蛋白表达量、Smad3磷酸化程度显著升高(P<0.05);与背部创伤组相比,紫榆膏处理组、凡士林处理组大鼠创面愈合率、胶原蛋白面积占比、血管面积占比显著升高,血清IL-6、IL-1β、TNF-α含量、TGF-β1 mRNA和蛋白表达量、Smad3磷酸化程度显著降低(P<0.05);与紫榆膏处理组相比,凡士林处理组、紫榆膏+TGF-β1激活组大鼠创面愈合率、胶原蛋白面积占比、血管面积占比显著降低,血清IL-6、IL-1β、TNF-α含量、TGF-β1 mRNA和蛋白表达量、Smad3磷酸化程度显著升高(P<0.05)。结论:紫榆膏通过调控TGF-β/Smad信号通路抑制相关基因和蛋白的表达,从而缓解炎症,促进创面愈合。Objective:To explore the effects of Ziyu Ointment on granulation tissue,TGF-βand downstream Smad protein in the process of wound healing in rats by constructing a rat model of back trauma.Methods:A rat model of back trauma was constructed,and 100 SD male rats were separated into healthy group,back trauma group,Ziyu Ointment treatment group,petrolatum treatment group,Ziyu Ointment+TGF-β1 activation group,with 20 per group.Wound formation surgery was performed,and wound healing of rats in each group was recorded at 5th,10th,and 15th day after operation;the levels of IL-6,IL-1βand TNF-αin the serum of rats in each group were detected;the granulation tissue formed by wound healing at 15th day was selected for HE and Masson staining,the proportion of blood vessel area and collagen area of granulation tissue of rats in each group were analyzed and calculated;the mRNA expression levels of TGF-β1 and Smad3 in granulation tissues of rats in each group were detected by fluorescence quantitative PCR;the expression levels of TGF-β1,Smad3 and p-Smad3 in granulation tissues of rats in each group were detected by Western blot.Results:The healthy group rats had complete epidermal tissue;the levels of serum IL-6,IL-1β,TNF-α,the expression of TGF-β1 mRNA and protein in the wound granulation tissue,and the phosphorylation of Smad3 in the back trauma group were obviously higher than the healthy group(P<0.05);compared with back trauma group,the wound healing rate,proportion of collagen area,and proportion of blood vessel area in Ziyu Ointment treatment group and petrolatum treatment group were obviously increased,the serum IL-6,IL-1β,TNF-αcontents,TGF-β1 mRNA and protein expression,and Smad3 phosphorylation were obviously decreased(P<0.05);compared with Ziyu Ointment treatment group,the petrolatum treatment group,Ziyu Ointment+TGF-β1 activation group had obviously lower wound healing rate,collagen area percentage,and blood vessel area percentage,and obviously lower serum IL-6,IL-1β,TNF-αcontents,TGF-β1 mRNA and protei

关 键 词：创面愈合 紫榆膏 肉芽组织 转化生长因子-Β SMAD蛋白 

分 类 号：R285.5[医药卫生—中药学]