青钱柳多糖通过降低类固醇5α还原酶2改善良性前列腺增生  

Cyclocarya paliurus Polysaccharide Inhibits Benign Prostatic Hyperplasia by Reducing 5α-Reductase 2

作　　者：代琴慧 闫孟霞 王晨 沈晨君 蒋晨莹 杨波[1] 赵华军[1] 朱智慧[1] DAI Qinhui;YAN Mengxia;WANG Chen;SHEN Chenjun;JIANG Chenying;YANG Bo;ZHAO Huajun;ZHU Zhihui(School of Pharmaceutical Sciences,Zhejiang Chinese Medical University,Hangzhou 310053,China)

机构地区：[1]浙江中医药大学药学院,杭州310053

出　　处：《中国实验方剂学杂志》2025年第3期107-114,共8页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金青年基金项目(82104448);浙江中医药大学校级人才专项(2021ZR10)。

摘　　要：目的:探讨青钱柳多糖(CPWP)抑制良性前列腺增生(BPH)的作用及机制研究。方法:采用加热回流、水提醇沉、冷冻干燥等方法提取CPWP。通过1-苯基-3-甲基-5-吡唑啉酮柱前衍生高效液相色谱法、红外光谱法等分析CPWP的化学组成和结构特性。将雄性SD大鼠随机分为正常组、模型组、非那雄胺组及CPWP低、中、高剂量组,每组8只。模型组和实验组每日分别以10 mg·kg^(-1)剂量腹腔注射(ip)丙酸睾酮造前列腺增生大鼠模型,非那雄胺组及CPWP低、中、高剂量组同时以5、50、75、100 mg·kg^(-1)剂量灌胃(ig)给药,正常组每日ip同体积的生理盐水,连续给药30 d。给药结束后,杀检大鼠,分离并称量前列腺组织,观察药物对大鼠体质量、前列腺湿重及前列腺指数影响。采用苏木素-伊红(HE)染色前列腺组织进行病理观察,酶联免疫吸附测定法(ELISA)检测二氢睾酮(DHT)水平,免疫组化染色检测前列腺组织中类固醇5α还原酶2(SRD5A2)和Ki67表达。结果:经鉴定,CPWP为糖类物质,具有糖类化合物特征吸收峰,总糖含量约为44.15%,相对分子质量主要分布于5.5~78.8 kDa,由甘露糖、鼠李糖、半乳糖醛酸、葡萄糖、半乳糖、木糖、阿拉伯糖等组成。药效学评价结果发现,与正常组比较,模型组大鼠前列腺湿质量和指数显著升高(P<0.01),前列腺柱状上皮细胞增生较厚且变高大、部分内褶皱增多呈乳头状向腔内扩张,血清中二氢睾酮及前列腺组织中SRD5A2、Ki67表达明显升高(P<0.05,P<0.01);与模型组比较,非那雄胺组和CPWP各剂量组前列腺湿重、指数下降(P<0.05,P<0.01),前列腺上皮细胞厚度减少、仅少部分内褶皱呈乳头状向腔内扩张,乳头突起长度降低,血清中DHT水平及前列腺组织中SRD5A2、Ki67表达显著降低(P<0.01),且CPWP具有剂量依赖性。结论:CPWP对大鼠良性前列腺增生具有抑制作用,作用机制与抑制SRD5A2的表达相关。Objective:To investigate the effect and mechanism of polysaccharide in water extract of Cyclocarya paliurus(CPWP)in inhibiting benign prostatic hyperplasia(BPH).Methods:CPWP was obtained by heating reflux,aqueous extraction,alcohol precipitation,and freeze drying.The chemical composition and structural properties of CPWP were analyzed by high performance liquid chromatography with 1-pheny-3-methyl-5-pyrazolone pre-column derivatization and infrared spectroscopy.Male SD rats were randomly assigned into control,model,finasteride(ig 5 mg·kg^(-1)),and low-,medium-,and high-dose(ig 50,75,100 mg·kg^(-1))CPWP groups,with 8 rats in each group.The BPH model was established by subcutaneously injecting propionate testosterone in castrated rats.The rats in the drug intervention groups were administrated with corresponding drugs,and those in the control group were administrated with an equal volume of normal saline each day.After 30 consecutive days,the rats were sacrificed,and the prostate tissue was separated and weighed.The effects of drug interventions on the body weight,prostate wet weight,and prostate index of rats were examined.The prostate tissue was stained with hematoxylin-eosin(HE)for observation of pathological changes.Enzyme-linked immunosorbent assay was employed to measure the level of dihydrotestosterone(DHT),and immunohistochemical staining was used to detect the expression of steroid 5 alpha-reductase 2(SRD5A2)and Ki67 in the prostate tissue.Results:CPWP was identified as a saccharide,with characteristic absorption peaks of saccharides.CPWP showed the total sugar content of 44.15%and molecular weight within the range of 5.5-78.8 kDa,being composed of mannose,rhamnose,galacturonic acid,glucose,galactose,xylose,and arabinose.Compared with the control group,the model group had significantly increased prostate wet weight and prostate index(P<0.01),thick and tall prostate epithelial cells,increased internal wrinkles,papillary expansion into the cavity,an elevation in DHT level in the serum,and up-regulated expr

关 键 词：良性前列腺增生 青钱柳多糖 二氢睾酮 类固醇5α还原酶2(SRD5A2) KI67 

分 类 号：R285[医药卫生—中药学] R243[医药卫生—中医学] R259