运脾益肾通督方对强直性脊柱炎模型小鼠脊柱病变及免疫炎症的影响  

Effect of Yunpi Yishen Tongdu Decoction on Spinal lesion and Immune Inflammation of Ankylosing Spondylitis Model Mice

作　　者：邱富海 刘彬 邱一吾 汪曼莉 陈慧楠 何志兴[1] 毛盈颖 QIU Fuhai;LIU Bin;QIU Yiwu(Institute of Clinical Fundamentals and Immunology of Traditional Chinese Medicine,School of Basic Medical Sciences,Zhejiang Chinese Medical University,Hangzhou(310053),China;School of Public Health,Zhejiang Chinese Medical University)

机构地区：[1]浙江中医药大学基础医学院中医临床基础与免疫研究院,杭州310053 [2]浙江中医药大学公共卫生学院

出　　处：《浙江中医药大学学报》2025年第1期1-8,共8页Journal of Zhejiang Chinese Medical University

基　　金：国家自然科学基金项目(81973663)。

摘　　要：[目的]明确运脾益肾通督方对强直性脊柱炎(ankylosing spondylitis,AS)小鼠脊柱病变及免疫炎症的影响,为临床治疗AS提供新的科学依据。[方法]42只雌性BALB/c小鼠随机分为空白组,模型组,西药组和低、中、高剂量运脾益肾通督方组。除空白组外,其余5组小鼠均在腹腔部注射蛋白聚糖构建AS模型。造模结束后,空白组和模型组小鼠灌胃0.9%氯化钠溶液,西药组小鼠灌胃3.57 mg·mL-1的塞来昔布溶液,低、中和高剂量运脾益肾通督方组小鼠分别灌胃0.9、1.8和3.6 mg·mL-1的运脾益肾通督方水煎剂,持续4周。灌胃结束后,检测各组小鼠行为学变化、血清炎症因子表达、脊柱病变及脊柱破骨细胞分化因子(receptor activator of nuclear factor-κB ligand,RANKL)、核转录因子活化受体-κB(receptor activator of nuclear factor-κB,RANK)和骨保护素(osteoclastogenesis inhibitory factor,OPG)的表达情况。[结果]与空白组比较,模型组小鼠的悬尾不动总时间、血清白细胞介素-6(interleukin-6,IL-6)和肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)水平、脊柱病理评分、RANKL和RANK表达显著升高(P<0.01,P<0.05),而脊柱椎体骨密度和OPG表达显著下降(P<0.01)。运脾益肾通督方治疗后,AS小鼠的悬尾不动潜时缩短、血清IL-6和TNF-α水平以及脊柱病理评分显著下降(P<0.01,P<0.05),RANKL/RANK表达下调,OPG表达上调(P<0.01,P<0.05)。[结论]运脾益肾通督方通过影响RANKL/RANK/OPG通路,缓解AS的骨破坏。[Objective Objective]To explore the alleviating effect of Yunpi Yishen Tongdu Decoction on spinal lesion and immune inflammation of ankylosing spondylitis(AS)model mice,and to provide a scientific basis for its clinical application.[Methods Methods]A total of 42 female BALB/c mice were randomly divided into blank group,model group,western medicine group,low,medium and high dose Yunpi Yishen Tongdu Decoction groups.Except for blank group,the remaining 5 groups were injected with proteoglycans in the abdominal cavity to construct AS model.After modeling,the mice in blank group and model group were given 0.9%sodium chloride solution,the mice in western medicine group were given 3.57 mg·mL-1 celecoxib solution,and the mice in the low,medium,and high dose Yunpi Yishen Tongdu Decoction were given 0.9,1.8 and 3.6 mg·mL-1 of Yunpi Yishen Tongdu Decoction for 4 weeks,respectively.After gavage,the behavioral changes,serum inflammation cytokines,spinal lesions,and expression of receptor activator of nuclear factor-κB ligand(RANKL),receptor activator of nuclear factor-κB(RANK),and osteoclastogenesis inhibitory factor(OPG)in the spine were measured.[Results Results]Compared with blank group,model group had significantly increased total time in tail immobility,serum interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)level,spinal pathological score,RANKL and RANK expression(P<0.01,P<0.05),while the vertebral bone mineral density and OPG expression of the spine were significantly decreased(P<0.01).After treatment with the Yunpi Yishen Tongdu Decoction,the latency time in tail immobility in AS mice was significantly reduced,along with decreased serum levels of IL-6 and TNF-αand a notable reduction in spinal pathological scores(P<0.01,P<0.05).Additionally,the treatment downregulated RANKL and RANK expression,upregulated OPG expression(P<0.01,P<0.05).[Conclusion Conclusion]Yunpi Yishen Tongdu Decoction alleviates the bone destruction of AS by affecting the RANKL/RANK/OPG pathway.

关 键 词：强直性脊柱炎 运脾益肾通督方 蛋白聚糖 RANKL/RANK/OPG通路 炎症 免疫性疾病 骨破坏 中药 

分 类 号：R331[医药卫生—人体生理学]