长链非编码RNA转移相关肺腺癌转录本1在糖尿病心肌病大鼠中的表达及意义  

Effects and mechanism of HMG CoA reductase inhibitor on diabetes cardiomyopathy based on LncRNA

作　　者：李丽芳[1] 王琴[2] 孙永福[3] 杨中男 蔡艳 田志州 海瑞 李燕萍[1] LI Lifang;WANG Qin;SUN Yongfu;YANG Zhongnan;CAI Yan;TIAN Zhizhou;HAI Rui;LI Yanping(Department of General Medicine,the First People's Hospital of Yinchuan,Yinchuan 750001,China;Zhongshan South Street Community Health Service Center,the First People's Hospital of Yinchuan,Yinchuan 750001,China;Department of Medical Laboratory,the First People's Hospital of Yinchuan,Yinchuan 750001,China;Department of Rehabilitation,the First People's Hospital of Yinchuan,Yinchuan 750001,China;Department of Cardiovascular Medicine,People's Hospital of Pengyang,Guyuan 756599,China;Tanshan Health Center of Yuanzhou District,Guyuan 756005,China)

机构地区：[1]宁夏银川市第一人民医院全科医学科,宁夏银川750001 [2]宁夏银川市第一人民医院中山南街社区卫生服务中心,宁夏银川750001 [3]宁夏银川市第一人民医院检验科,宁夏银川750001 [4]宁夏银川市第一人民医院康复医院康复科,宁夏银川750001 [5]宁夏彭阳县人民医院人民心血管内科,宁夏固原756599 [6]宁夏固原市原州区炭山乡卫生院,宁夏固原756005

出　　处：《宁夏医学杂志》2025年第1期12-15,F0003,共5页Ningxia Medical Journal

基　　金：宁夏自然科学基金项目(2022AAC03742)。

摘　　要：目的探讨3-羟基-3甲基戊二酰辅酶A(HMG-CoA)还原酶抑制剂通过长链非编码RNA转移相关肺腺癌转录本1(LncRNA MALATl)对糖尿病心肌病的作用及机制。方法选取正常SD大鼠30只(雄性,体重220~260 g),将其分为正常组(n=10)和糖尿病模型组(n=20)。糖尿病模型组给予注射链脲佐菌建立糖尿病心肌病模型后,分为糖尿病心肌病组(n=10)、阿托伐他汀组(n=10)。阿托伐他汀组给予阿托伐他汀灌胃干预治疗,正常组、糖尿病心肌病组采用等量生理盐水灌胃,12周后注射25%乌拉坦麻醉后处死大鼠,留取外周血,制备心肌病理标本及心肌匀浆。用TUNEL法检测心肌凋亡细胞,用荧光定量PCR法检测外周血及心肌组织中LncRNA MALATl水平。结果糖尿病心肌病组大鼠心肌组织细胞肥大,多数细胞的胞浆内可见粉染颗粒,间质血管扩张充血,并有少量点灶状出血。与糖尿病心肌病组相比,阿托伐他汀组大鼠心肌组织细胞损伤明显改善;与正常组比较,糖尿病心肌病组大鼠细胞凋亡数增加,阿托伐他汀组能够明显降低大鼠心肌细胞的凋亡数;LncRNA MALATl在糖尿病心肌病组、阿托伐他汀组外周血及心肌组织中表达明显升高,与正常组比较差异有统计学意义(P<0.05),糖尿病心肌病组、阿托伐他汀组组间比较差异无统计学意义(P>0.05)。结论LncRNA MALATl在糖尿病心肌病大鼠中异常表达,HMG-CoA还原酶抑制剂具有抑制糖尿病心肌病大鼠心肌细胞损伤、细胞凋亡的作用,可能不具有抑制LncRNA MALATl的作用。Objective To investigate the effect and mechanism of 3-hydroxy-3-methylglutaryl Coenzyme A(HMG-CoA)reductase inhibitors on diabetic cardiomyopathy through long non-coding RNA transfer associated lung adenocarcinoma transcript 1(LncRNA MALATl).Methods 30 SD rats(male,220 g~260 g)were divided into normal group(n=10)and diabetic group(n=20).The diabetic group was administered streptozotocin through one-time intraperitoneal injection to establish the model of diabetes cardiomyopathy,and then further divided into diabetes cardiomyopathy group(n=10)and atorvastatin group(n=10).The atorvastatin group was given atorvastatin by gavage,and the normal group and the diabetic cardiomyopathy group were given equal volume of normal saline by gavage.After 12 weeks,25% urethane was injected and the rats were killed.Peripheral blood was retained and the myocardial pathological specimens and myocardial homogenates were prepared.TUNEL method was used to detect myocardial apoptosis,and fluorescent quantitative PCR method was used to detect the levels of LncRNA MALATl in peripheral blood and myocardial tissue.Results The myocardial tissue cells of rats in the diabetic cardiomyopathy group showed edema.The cytoplasm of most cells showed pink particles,interstitial blood vessels were dilated and congested,and a small amount of focal bleeding.Compared with the diabetic cardiomyopathy group,and cellular damage of myocardial tissue was improved in atorvastatin group.There were fewer apoptotic cells in the myocardium of rats in the atorvastatin group than those in the diabetic cardiomyopathy group.The expression of LncRNA MALATl in peripheral blood and myocardial tissue of diabetic cardiomyopathy group and atorvastatin group was significantly higher than that of normal group(P<0.05),and there was no difference between the rats in the diabetic cardiomyopathy group and atorvastatin group(P>0.05).Conclusion LncRNA MALATl is abnormally expressed in diabetes cardiomyopathy rats.HMG-CoA reductase inhibitor can inhibit the damage and apoptosis of

关 键 词：长链非编码RNA转移相关肺腺癌转录本1 阿托伐他汀 糖尿病心肌病 大鼠 

分 类 号：R587.2[医药卫生—内分泌]