青海草原毛虫雌蛾性腺气味受体基因的鉴定及组织表达分析  

Identification and tissue expression profiling of odorant receptor genes in sex pheromone glands of female Gynaephora qinghaiensis(Lepidoptera:Lymantriidae)

作　　者：刘占玲 高书晶[2] 韩海斌[2] 唐德靖 孔一森 潘学能 周渊涛 LIU Zhanling;GAO Shujing;HAN Haibin;TANG Dejing;KONG Yisen;PAN Xueneng;ZHOU Yuantao(School of Agriculture and Animal Husbandry,Qinghai University,Xining 810016,China;Institute of Grassland Research,Chinese Academy of Agricultural Sciences,Hohhot 010010,China)

机构地区：[1]青海大学农牧学院,西宁810016 [2]中国农业科学院草原研究所,呼和浩特010010

出　　处：《植物保护》2025年第1期69-79,共11页Plant Protection

基　　金：国家重点研发计划(2022YFD1401101)。

摘　　要：为明确青海草原毛虫Gynaephora qinghaiensis雌蛾性腺气味受体(odorant receptors,ORs)基因的分子特性及组织表达水平,进而为后续基因功能的研究奠定基础,本研究利用生物信息学方法及RT-qPCR技术,对性腺ORs基因进行鉴定及表达谱分析。从性腺转录组数据库共筛选鉴定出13个气味受体基因(包括12个GqinORs,1个GqinOR_(co)),其中只有GqinOR1具有1个信号肽;除GqinOR3、GqinOR7和GqinOR9以外,其余GqinORs具有1~3个跨膜结构域,GqinOR_(co)具有8个跨膜结构;GqinORs二级结构以α螺旋为主。系统进化分析表明,GqinORs可能与桃蛀螟Conogethes punctiferalis和小线角木蠹蛾Streltzoviella insularis的ORs具有共同祖先。基因表达谱分析发现,性腺ORs基因在雌雄不同组织表现出不同的表达水平(P<0.05),13个气味受体基因在性腺中都有表达,且GqinOR8、GqinOR10在触角和性腺中高表达。本研究为进一步研究青海草原毛虫气味受体嗅觉功能提供重要依据。To clarify the molecular characteristics and tissue-specific expression levels of odorant receptor(OR)genes in the sex pheromone glands of female Gynaephora qinghaiensis,this study employed bioinformatics and RT-qPCR techniques for gene identification and expression profile analysis.A total of 13 OR genes were screened and identified from the pheromone gland transcriptome database,including 12 GqinORs and 1 GqinOR_(co).Only GqinOR1 had a signal peptide.Most GqinORs,except GqinOR3,GqinOR7 and GqinOR9,had one to three transmembrane domains,while GqinOR_(co)had eight transmembrane domains.The secondary structure of GqinORs is mainly composed ofαhelices.Phylogenetic analysis showed that GqinORs may share a common ancestor with ORs from Conogethes punctiferalis,and Streltzoviella insularis.Gene expression profile analysis showed that the expression levels of OR genes in different male and female tissues were significantly different(P<0.05),with all 13 target genes expressed in sex pheromone glands,and GqinOR 8 and GqinOR 10 were highly expressed in the antennae and sex pheromone glands.This study provides an important basis for further research on olfactory functions in the odorant receptors of G.qinghaiensis.

关 键 词：青海草原毛虫 气味受体 性腺 生物信息学分析 表达谱分析 

分 类 号：S812.6[农业科学—草业科学]