日本平腹小蜂滞育幼虫的表型研究  

Phenotypic study on diapause larvae of Anastatus japonicus Ashmead

作　　者：肖俊健 袁龙宇 夏玥 李敦松[1] 赵灿[1] XIAO Junjian;YUAN Longyu;XIA Yue;LI Dunsong;ZHAO Can(Institute of Plant Protection,Guangdong Academy of Agricultural Sciences,Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China,Ministry of Agriculture and Rural Affairs,Guangdong Provincial Key Laboratory of High Technology for Plant Protection,Guangzhou 510640,China;State Key Laboratory of Green Pesticide,College of Plant Protection,South China Agricultural University,Guangzhou 510642,China)

机构地区：[1]广东省农业科学院植物保护研究所,农业农村部华南果蔬绿色防控重点实验室,广东省植物保护新技术重点实验室,广州510640 [2]华南农业大学植物保护学院,绿色农药全国重点实验室,广州510642

出　　处：《植物保护》2025年第1期89-94,101,共7页Plant Protection

基　　金：国家重点研发计划(2023YFD1400600);国家自然科学基金青年基金(32001968);国家荔枝龙眼产业技术体系(CARS-32-09);广东省科技计划(2023B0202010010);广东省农业科学院“青年研究员项目”(R2023PY-QY007)。

摘　　要：日本平腹小蜂Anastatus japonicus Ashmead是多种蝽类害虫的优势卵寄生蜂,以老熟幼虫滞育。本研究比较了日本平腹小蜂非滞育、滞育、滞育解除老熟幼虫的体重、体长、体宽及体色的变化,并通过尼罗红染色来观察滞育幼虫体内脂滴的变化。结果表明:滞育期间老熟幼虫体重逐渐减轻,体长逐渐缩短,体宽无显著变化,体色灰度值呈现缓慢降低的趋势;相较于非滞育个体,随着滞育时间的延长,滞育老熟幼虫体腔内脂滴数量逐渐减少,同时出现表皮附近脂滴聚集现象,表明脂质是日本平腹小蜂滞育期间主要的能源物质,随着滞育时间延长,脂质被逐渐消耗。而脂滴向日本平腹小蜂老熟幼虫表皮附近大量聚集,有助于增强滞育个体的抗冻能力。本文为深入研究日本平腹小蜂滞育机制奠定基础。Anastatus japonicus Ashmead is a dominant egg parasitic wasp for various bug pests,entering diapause as mature larvae.In this study,the body weight,body length,body width and body color of mature larvae of A.japonicus in non-diapause,diapause and diapause-terminated states were measured.Additionally,the changes of lipid droplets within the larvae were observed using Nile red staining.The results showed a gradual decrease in body weight and grayscale value of body color during diapause,while the body length shortened with no significant change in body width.Compared to non-diapause larvae,the lipid droplets in the body cavity of diapause larvae gradually decreased with the prolongation of diapause period,along with an accumulation of lipid droplets near the body surface.These results indicate that lipids serve as the main energy source during diapause of A.japonicus,and are progressively consumed over time.The accumulation of lipid droplets near the larval body surface likely aids in enhancing the freezing resistance of diapause individuals.This study lays a foundation for further research into the diapause mechanism of A.japonicus.

关 键 词：日本平腹小蜂 滞育 生物学表型 脂滴 

分 类 号：S436.67[农业科学—农业昆虫与害虫防治]