云南芒果枯萎病病原菌鉴定  

Identification of the pathogen causing mango wilt disease in Yunnan

作　　者：张治萍 张竹青 窦敏 范福园 任云贵 刘波 沙素飞 李健强 ZHANG Zhiping;ZHANG Zhuqing;DOU Min;FAN Fuyuan;REN Yungui;LIU Bo;SHA Sufei;LI Jianqiang(College of Landscape and Horticulture,Yunnan Agricultural University,Kunming 650201,China;College of Plant Protection,Yunnan Agricultural University,Kunming 650201,China;College of Plant Protection,China Agricultural University,Beijing 100193,China)

机构地区：[1]云南农业大学园林园艺学院,昆明650201 [2]云南农业大学植物保护学院,昆明650201 [3]中国农业大学植物保护学院,北京100193

出　　处：《植物保护》2025年第1期141-148,共8页Plant Protection

基　　金：国家自然科学基金(31571958);云南省李健强专家工作站(202105AF150046);云南省“兴滇英才支持计划”青年人才(XDYC-QNRC-2022-0710);云南农业大学科研启动基金(KY2022-64)。

摘　　要：2016年-2022年,采集云南省临沧市永德县主要芒果种植基地疑似枯萎病样品60份,通过对疑似样品的分离、纯化,结合形态学特征、多基因系统发育分析及柯赫氏法则验证,对8株代表性分离物(MG-1-1,MG-1-3,MG-1-7,MG-1-10,MG-2-1,MG-2-4,MG-2-8,MG-2-10)进行鉴定。结果表明:8株分离物在MYEA培养基上散发出果香味,形成大量子囊壳和子囊孢子团,子囊孢子帽盔状;序列同源性分析显示:分离物的βT1、RPBII、MS204和FG1093序列与长喙壳属真菌Ceratocystis manginecans菌株序列相似性为100%,4个基因片段串联构建系统发育树显示,8株分离物与源自阿曼和印度尼西亚的C.manginecans聚为一支,ML和BI分析支持率分别为93和0.98。选取2个分离物(MG-1-10和MG-2-8)进行致病性测定,均能引起芒果幼苗发病且症状与田间相似。综上,本研究明确了引起云南芒果枯萎病的病原菌为Ceratocystis manginecans。From 2016 to 2022,sixty suspected mango wilt disease samples were collected from the main mango cultivation base in Yongde county,Lincang city,Yunnan province.Using pathogen isolation,purification,morphological characterization,multi-gene phylogenetic analysis,and Koch’s postulates verification,eight representative isolates(MG-1-1,MG-1-3,MG-1-7,MG-1-10,MG-2-1,MG-2-4,MG-2-8 and MG-2-10)were identified.All isolates emitted a fruity aroma on MYEA medium,formed prominent perithecia and ascospore masses,and displayed hat-shaped ascospores.Sequence homology analysis revealed 100%similarity inβT1,RPBII,MS204 and FG1093 gene sequences between isolates and Ceratocystis manginecans strains from Oman and Indonesia.A phylogenetic tree constructed from these gene sequences grouped the eight isolates with C.manginecans with a bootstrap support of 93 and a Bayesian support value of 0.98.Pathogenicity tests on mango seedlings using isolates MG-1-10 and MG-2-8 revealed that they caused symptoms consistent with those observed in the field.This study thus confirms C.manginecans as the pathogen responsible for mango wilt disease in Yunnan.

关 键 词：芒果 枯萎病 病原鉴定 Ceratocystis manginecans 

分 类 号：S432.1[农业科学—植物病理学]