单链DNA退火蛋白介导细菌基因组同源重组的机制及应用研究进展  

Mechanisms and Application Research Progress of Bacterial Genomic Homologous Recombination Mediated by Single-stranded DNA Annealing Protein

作　　者：尹号 尤留超 韩瑞 高鹏程[1,2] 付磊 储岳峰 YIN Hao;YOU Liu-chao;HAN Rui;GAO Peng-cheng;FU Lei;CHU Yue-feng(State Key Laboratory for Animal Disease Control and Prevention,College of Veterinary Medicine,Lanzhou University,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730000;Gansu Province Research Center for Basic Disciplines of Pathogen Biology,Lanzhou 730046)

机构地区：[1]中国农业科学院兰州兽医研究所兰州大学动物医学与生物安全学院动物疫病防控全国重点实验室,兰州730000 [2]甘肃省病原生物学基础学科研究中心,兰州730046

出　　处：《生物技术通报》2025年第1期39-48,共10页Biotechnology Bulletin

基　　金：国家自然科学基金面上项目(32373019);中国农业科学院兰州兽医研究所重点项目(CAAS-ASTIP-JBGS-20210701);中国农业科学院兰州兽医研究所基本业务费-科研业务费项目(110231160042012);甘肃省重点人才项目(2021RCXM047);甘肃省陇原青年创新创业人才(个人)项目(2023LQGR55)。

摘　　要：基因组编辑技术是研究细菌等微生物在基因功能、耐药机制及致病机制等方面的重要工具,而同源重组是细菌基因组编辑的重要方式之一,传统的细菌内源性重组途径存在效率低下的问题,而一种来自噬菌体的单链DNA退火蛋白(SSAP)表现出了远超内源性重组途径的基因组编辑效率,该蛋白具有单链DNA结合活性、介导基因组定向重组的特点,使其成为目前极具潜力的基因组编辑工具。本文主要对同源重组基本原理、噬菌体源SSAP介导的同源重组途径的基本元件、重组机制模型以及应用策略展开概述,旨在为进一步解析单链DNA退火蛋白介导的同源重组过程提供帮助,为研究更多细菌基因功能、致病机制以及开发工程菌株提供技术支撑,也为缺乏基因编辑方法的细菌提供技术参考。Genome editing technology is an important tool for studying the gene function,drug resistance mechanism,and pathogenic mechanism of bacteria and other microorganisms.Homologous recombination is one of the key methods for bacterial genome editing.The conventional endogenous pathways in bacteria suffer from low efficiency.However,a single-stranded DNA annealing protein(SSAP)from bacteriophages,which has shown gene editing efficiencies far surpassing those of endogenous pathways.This protein has the characteristics of single-stranded DNA binding activity and mediates genome directed recombination,making it an extremely promising tool for genome editing.This article provides an overview of the basic principles of homologous recombination,the fundamental components of the SSAP-mediated homologous recombination pathway from phages,recombination mechanism models,and application strategies.The aim is to assist in further elucidating the process of homologous recombination mediated by SSAP to provide technical support for studying the functions and pathogenic mechanisms of more bacterial genes,and to develop engineering strains.It also provides technical support for bacteria lacking gene editing methods.

关 键 词：细菌 单链DNA退火蛋白 同源重组 机制 应用 

分 类 号：Q78[生物学—分子生物学]