藜麦MADS-box基因家族的全基因组鉴定和表达分析  

Genome-wide Identification and Expression Analysis of the MADS-box Gene Family in Quinoa

作　　者：何财林 卢晶 郭会会 李小安 吴琪[1,2] HE Cai-lin;LU Jing;GUO Hui-hui;LI Xiao-an;WU Qi(Key Laboratory of Coarse Cereal Processing,Ministry of Agriculture and Rural Affairs,College of Food and Biological Engineering,Chengdu University,Chengdu 610106;Sichuan Engineering&Technology Research Center of Coarse Cereal Industrialization,College of Food and Biological Engineering,Chengdu University,Chengdu 610106)

机构地区：[1]农业农村部杂粮加工重点实验室成都大学食品与生物工程学院,成都610106 [2]四川省杂粮产业化工程技术研究中心成都大学食品与生物工程学院,成都610106

出　　处：《生物技术通报》2025年第1期157-172,共16页Biotechnology Bulletin

基　　金：西南作物基因资源发掘与利用国家重点实验室开放课题(SKL-KF202302);四川省自然科学基金项目(2024NSFSC0329);西藏自治区农牧科学院农业研究所项目(NYSTC202408)。

摘　　要：【目的】开展藜麦CqMADS-box基因家族鉴定和表达分析,为藜麦产量品质的提高提供优异基因资源。【方法】基于拟南芥MADS-box蛋白序列,利用BLASTP和隐马尔可夫模型文件(Hidden Markov Model, HMM)鉴定CqMADS-box基因家族成员,采用最大似然法(maximum likelihood, ML)构建系统发育树,分析MADS-box基因在染色体的位置,利用MXSCan分析CqMADSbox基因家族成员在藜麦亚基因组A/B间,及其与甜菜二倍体近缘种C. pallidicaule(A)、C. suecicum(B),六倍体近缘种中国台湾红藜(Chenopodium formosanum)中MADS-box成员的共线性,利用WoLF PSORT、PlantCARE等在线网站分析CqMADS-box转录因子的理化性质、基因结构、启动子区顺式作用元件。基于前期发表的转录组数据,分析CqMADS-box在不同组织、6个花序发育时期、种子萌发的表达模式;利用转录组数据分析在长日照和短日照下,花期和花器官相关CqMADS-box的表达情况。【结果】鉴定得到117个藜麦MADS-box基因家族成员,分布在17条染色体上。Ⅰ型CqMADS-box包含Mα、Mγ两个进化分支,Ⅱ型包含CqPI(CqGLO)、CqAGL17等14个进化分支。不同分支CqMADS-box基因结构、蛋白Motif组成、转录因子结合位点类型和数量存在不同程度的差异。进化分析表明,串联复制是藜麦MADS-box家族扩张的主要途径,大部分复制的时间在四倍体化之前。表达模式结果表明,CqMADS-box基因在花序中表达量相对较高,而在花序发育前期(YP1或YP2),CqSEP3、CqAP1、CqGGM13类基因呈相对高表达;种子经过ABA处理后,CqSEP3类基因表达量显著上升;不同光周期下的表达模式表明,CqAP1、CqSOC1类基因在长日照和短日照下表达量均较高。【结论】CqMADS-box基因家族成员具有组织特异性表达模式,且CqSEP3类基因在藜麦花序发育和响应ABA激素处理的过程中发挥重要作用。【Objective】Identification and expression analysis of CqMADS-box gene family in quinoa(Chenopodium quinoa)were carried out to provide excellent gene resources for improving the yield and quality of quinoa.【Method】Based on the MADS-box protein sequences of Arabidopsis thaliana,BLASTP and Hidden Markov model(HMM)were employed to identify the members of the CqMADS-box gene family,and maximum likelihood method(ML)was to construct the phylogenetic tree,and analyze the positions of MADS-box genes on the chromosomes.MXSCan was used to analyze the collinear relationships of MADS-box family members between quinoa sub-genome A/B and their relatives sugar beet(Beta vulgaris)and diploid C.pallidicaule(A),C.suecicum(B)and hexaploid species Chenopodium formosanum.Online tools WoLF PSORT and PlantCARE were utilized to analyze the physicochemical properties,gene structure,and cis-acting elements of CqMADS-box transcription factors.Based on our previous transcriptome data,the expression patterns of CqMADS-box were analyzed in different tissues,six inflorescence development stages and during seed germination.The expression patterns of CqMADS-box related to flowering and flower organs were analyzed under long-day and short-day conditions.【Result】A total of 117 members of the MADS-box gene family were identified on 17 chromosomes.CqMADS-box genes of type I contained two subfamilies,Mαand Mγ,and type II CqMADS-box genes contained 14 subfamilies of CqPI(CqGLO)and CqAGL17.There were differences in the CqMADS-box gene structure,protein Motif composition,and transcription factor binding site types and numbers in different branches.Evolutionary analysis showed that tandem replication was the major driving force for MADS-box family expansion in quinoa,and most of the duplication events occurred before the tetraploidization.Gene expression pattern analysis showed that the overall expressions of CqMADS-box genes were relatively higher in the inflorescence,while the expressions of CqSEP3,CqAP1 and CqGGM13 genes were relatively hi

关 键 词：CqMADS-box 藜麦 全基因组鉴定 进化分析 花序发育 光周期 

分 类 号：Q943.2[生物学—植物学] S519[农业科学—作物学]