慢性乙肝患者HBV-DNA载量、肝功能及肝纤维指标检测分析  

Detection and analysis of Th1/Th2 cytokines and liver fiber indicators in hepatitis B patients during antiviral treatment

作　　者：吴秀君 程海霞 吕晓丹 Wu Xiu-jun;Cheng Hai-xia;Lv Xiao-dan(Wuyuan Traditional Chinese Medicine Hospital Laboratory,Shangrao 333200,Jiangxi,China)

机构地区：[1]婺源县中医院检验科,江西上饶333200

出　　处：《四川生理科学杂志》2025年第1期186-188,共3页

摘　　要：目的:研究慢性乙肝脱氧核糖核酸(HBV-DNA)与其肝功能及肝纤维指标的关系。方法:选择我院70例HBV患者为高水平组,采集时间为2021年1月-2023年12月,按照HBV-DNA载量水平分为低水平组与高水平组,均于入院时采用酶联免疫吸附法检测两组谷丙转氨酶(ALT)、谷草转氨酶(AST)、γ-谷氨酰基转移酶(GGT);采用放射免疫法检测肝纤维指标[透明质酸(HA)、层粘连蛋白(LN)、Ⅲ型前胶原肽(PC Ⅲ)];比较各组肝功能指标、纤维化指标,并分析HBV-DNA载量与肝功能及肝纤维指标的相关性。结果:高水平组血清AST、ALT、GGT、HA、LN、PC Ⅲ水平均高于低水平组,差异有统计学意义(P<0.05);Pearson相关性分析显示,HBV-DNA载量水平与AST、ALT、GGT、HA、LN、PC Ⅲ均呈正相关(P<0.05)。结论:慢性患者HBV-DNA载量与其肝功能及肝纤维指标存在明显关系,其HBV-DNA载量越高,AST、ALT、GGT、HA、LN、PC Ⅲ水平也就越高,临床可通过对上述指标的检测来判断患者疾病的严重程度。Objective:To study the changes of Th1/Th2 cytokines and liver fiber indicators in patients with hepatitis B virus(HBV)infection during antiviral treatment,and analyze the value of these indicators in efficacy evaluation.Methods Seventy HBV infected patients admitted to the hospital from January 2021 to December 2023 were selected as the observation group.Meanwhile,60 healthy individuals who received physical examinations in the hospital were selected as the normal control group.Serum Th1/Th2 cytokines[interferon-gamma(IFN-γ),interleukin-4(IL-4)and interleukin-6(IL-6)]in both groups were detected by enzyme linked immunosorbent assay.Liver fiber indicators[hyaluronic acid(HA),laminin(LN)and procollagen type Ⅲ(PC Ⅲ)]in the two groups were detected by radioimmunoassay.After receiving antiviral treatment with entecavir,the observation group was retested for Th1/Th2 cytokines and liver fiber indicators.Results The levels of IL-4,IL-6,HA,LN,and PC Ⅲ in the observation group were higher than those in the control group,and the level of IFN-γwas lower than that in the control group(P<0.05).After treatment,the levels of IL-4,IL-6,HA,LN,and PC Ⅲ in the observation group decreased,and the level of IFN-γincreased(P<0.05).Conclusion There is significant imbalance in Th1/Th2 cytokines and liver fiber indicators in HBV infected patients,compared with normal individuals,which is characterized by elevated levels of IL-4,IL-6,HA,LN and PC Ⅲ,and decreased IFN-γlevel.Antiviral treatment with entecavir can significantly improve the levels of Th1/Th2 cytokines and liver fibrosis indicators.Based on above results,clinical efficacy of antiviral treatment can be judged by detecting these indicators.

关 键 词：慢性乙肝 HBV-DNA载量 肝功能 肝纤维指标 

分 类 号：R512.62[医药卫生—内科学]