等温扩增技术检测人乳头瘤病毒的研究进展  

Progress in the detection of human papilloma virus by isothermal amplification technology

作　　者：李泓熠 马学军[3] 帖彦清[2] 申辛欣[3] Li Hongyi;Ma Xuejun;Tie Yanqing;Shen Xinxin(Graduate School of Hebei North University,Hebei Zhangjiakou 075000,China;Laboratory Department of Hebei General Hospital,Hebei,Shijiazhuang 0500512,China;Key Laboratory of Medical Viruses and Viral Diseases,Chinese Center for Disease Control and Prevention,National Key Laboratory of Traceability and Early Warning of Infectious Diseases and Intelligent Decision Making,Beijing 102206,China)

机构地区：[1]河北北方学院研究生学院,河北张家口075000 [2]河北省人民医院检验科,河北石家庄0500512 [3]中国疾病预防控制中心病毒病预防控制所,传染病溯源预警与智能决策全国重点实验室,国家卫生健康委员会医学病毒和病毒病重点实验室,北京102206

出　　处：《新发传染病电子杂志》2024年第6期68-73,共6页Electronic Journal of Emerging Infectious Diseases

基　　金：国家自然科学基金(82202593);国家重点研发计划(2021YFC2301102);河北省重点研发计划(223777100D)。

摘　　要：宫颈癌是全球第四大女性癌症,几乎所有宫颈癌病例都与人乳头瘤病毒(human papilloma virus,HPV)感染有关,因此,HPV感染的早期发现对预防宫颈癌至关重要。WHO建议将HPV DNA检测作为主要筛查方法,等温扩增技术相较于变温扩增技术操作更简便,反应更高效,对实验条件,操作人员的要求更低,且经济成本更低,因此,更适用于HPV的检测。本文比对分析了用于HPV检测的等温扩增技术方法,常用的扩增产物判读方法及等温扩增技术与其他技术(如微流控芯片技术、CRISPR-Cas技术、智能手机检测和横向流动试纸条)结合检测的应用现状。对这些方法和技术进行优缺点分析,对其所适合的应用场景进行阐述,总结等温扩增技术存在的不足并对其发展前景进行展望。Cervical cancer is the fourth most common female cancer in the world.Almost all cases of cervical cancer are associated with human papilloma virus(HPV)infection,so early detection of HPV infection is essential for cervical cancer prevention.The World Health Organization recommended HPV DNA testing as the primary screening method.Isothermal amplification technology is more suitable for HPV testing because it is easier to operate and more efficient than variable temperature amplification technology,which requires less laboratory conditions,less operator effort,and lower economic costs.This paper comparatively analyses the advantages and disadvantages of isothermal amplification techniques used for HPV detection,commonly used amplification product readout methods and the current status of the application of isothermal amplification techniques in combination with other techniques(e.g.,microfluidic chip technology,CRISPR-Cas technology,smartphone assay,and lateral flow dipstick)for detection of HPV.At last,the advantages and disadvantages of these methods and techniques are analyzed,their suitable application scenarios are elaborated,and the shortcomings of isothermal amplification techniques are summarized and the prospects for their development are outlooked.

关 键 词：等温扩增技术 人乳头瘤病毒 研究进展 

分 类 号：R372[医药卫生—病原生物学]