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作 者:万建琦 丁瑜 任晗 李雯霞 杨勇[1] 黄金盟 关长飞 WAN Jianqi;DING Yu;REN Han;LI Wenxia;YANG Yong;HUANG Jinmeng;GUAN Changfei(College of Horticulture,Northwest A&F University,Yangling 712100,Shaanxi,China;Guangxi Academy of Specialty Crops,Guilin 541004,Guangxi,China)
机构地区:[1]西北农林科技大学园艺学院,陕西杨凌712100 [2]广西特色作物研究院,广西桂林541004
出 处:《果树学报》2025年第2期276-287,共12页Journal of Fruit Science
基 金:国家重点研发计划(2022YFD2200403);广西自然科学基金项目(2021GXNSFBA196032)。
摘 要:【目的】探究DdSOC1基因调控德阳柿(Diospyros deyangensis)成花的分子机制。【方法】通过生物信息学、基因表达量分析、酵母双杂筛库和双杂互作验证等方法,解析DdSOC1基因在调控成花中的功能。【结果】德阳柿DdSOC1序列和君迁子DlSOC1序列遗传距离较近;在成花诱导过程中,DdSOC1在茎、叶、芽中随开花进程出现差异表达;基于柿酵母文库,筛选获得7个DdSOC1的互作蛋白(MIOX、AGL14、JOINTLESS、GL2、NOVEIN、NBS、UBC7),酵母双杂交和双分子荧光互补试验表明,DdSOC1和上述7个蛋白均互作;qRT-PCR结果显示,一年生已开花的德阳柿SOC1、AGL14、JOINTLESS、NOVEIN、GL2、UBC7、NBS的表达量,幼叶高于成龄叶;二年生实生苗中,SOC1表达量差异不大,而AGL14、JOINTLESS、GL2、UBC7、MIOX表达量在开花实生苗中的表达量高于未开花实生苗。【结论】DdSOC1及其互作蛋白在德阳柿成花转变中发挥着重要作用,为探究德阳柿短童期分子调控网络提供了更多理论依据。【Objective】Seedlings of the new identified persimmon species Diospyros deyangensis have a short juvenile stage and can flower within one year after seed sowing.The function of the DdSOC1 gene in controlling flower bud initiation in D.deyangensis was investigated,along with its sequence characterization and expression pattern.【Methods】Plant materials were obtained from Nicotiana tabacum and 1-and 2-year-old persimmon(D.deyangensis)seedlings cultivated in the orchard at Northwest A&F University under natural conditions.Online analysis of the open reading frame of DdSOC1 was done using the ORF Finder.The physicochemical property of the encoded protein was predicted using Expasy ProtParam program.The MEGA7.0 software was used to create the phylogenetic tree.QRTPCR was used to examine the expression features of DdSOC1.On the one-deficient and three-deficient plates,the self-toxicity and self-activation of DdSOC1 were explored.Verification of the screening library and yeast interaction was done on a four-lack plate.Confocal microscopy was used in the BiFC experiment.【Results】The findings demonstrated that D.deyangnsis SOC1 shared a close genetic distance with SOC1 from Diospyros lotus,Actinidia chinensis,Actinidia eriantha,Camellia sinensis and other species.Populus alba,Vitis vinifera,Malus domestica,Prunus persica,Mangifera indica and other species were distantly linked to D.deyangnsis SOC1 protein.Furthermore,D.deyangnsis SOC1 protein was the most distantly related to that of the herbaceous plants Triticum aestivum and Oryza sativa.Online analysis demonstrated that DdSOC1 protein encodes 126 amino acids.The molecular weight is 14.56 ku.Its isoelectric point is 5.62.SOC1 expression was the highest during flowering and lowest during the bud stage in annual flowering D.deyangnsis stems.As the flowering process progressed,SOC1 expression in the leaves rose.SOC1 expression in the buds peaked during the seedling stage and decreased during the flowering stage.For Y2H,the first deficient plate had plaques,wherea
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