Effect of biochar on soil microbial community,dissipation and uptake of chlorpyrifos and atrazine  

作　　者：Raghvendra Pratap Singh Ranu Yadav Versha Pandey Anupama Singh Mayank Singh Karuna Shanker Puja Khare 

机构地区：[1]Crop Production and Protection Division,CSIR-Central Institute of Medicinal and Aromatic Plants,Lucknow 226015,India. [2]Phytochemistry Division,CSIR-Central Institute of Medicinal and Aromatic Plants,Lucknow 226015,India. [3]Academy of Scientific and Innovative Research(AcSIR),Ghaziabad 201002,India.

出　　处：《Biochar》2024年第1期271-287,共17页生物炭(英文)

基　　金：the Department of Biotechnology(DBT),New Delhi(BT/PR24706/NER/95/822/2017)under;the twinning program and CSIR-CIMAP(MLP-10)for financial assistance;CIMAP manuscript number is CIMAP/PUB/2023/141.

摘　　要：For the application of biochar in restoring pesticide-contaminated soils and minimizing the risk associated with their uptake in plants,it is crucial to understand the biochar impact on soil biological activities and dissipation and accumulation of pesticides in plant and soil systems.In this study,the effect of Mentha-distilled waste-derived biochar was investigated on chlorpyrifos and atrazine contaminated sandy loam soil.The four application rates of atrazine(2,4,6,and 8 mg kg^(−1))and chlorpyrifos(2,4,6,and 12 mg kg^(−1))and a single application rate of biochar(4%)were used in this study.The degradation of pesticides,the diversity of the bacterial community,and enzymatic activities(alkaline phosphatase,dehydrogenase,arylsulfatase,phenol oxidase,urease activity and N-acetyl glucosaminidase)were exam-ined in soil.The uptake of two pesticides and their effect on growth and stress parameters were also investigated in plants(A.paniculata).The dissipation of chlorpyrifos and atrazine followed simple first-order kinetics with a halflife of 6.6-74.6 and 21-145 days,respectively.The presence of deisopropyl atrazine desethyl atrazine(metabolites of atrazine)and 3,5,6-trichloro-2-pyridinol(a metabolite of chlorpyrifos)was observed in soil and plant tissues.Biochar application significantly(p=0.001)enhanced the degradation rate of chlorpyrifos and atrazine leading to the lower half-life of chlorpyrifos and atrazine in soil.A significant reduction(p=0.001)in the uptake of chlorpyrifos and atrazine and alteration in their binding affinity and uptake rate in plant tissues was observed in biochar treatments.The incorporation of biochar improved chlorpyrifos/atrazine degrader and plant growth-promoting bacterial genera such as Balneimonas,Kaistobacter,Rubrobacter,Ammoniphilus,and Bacillus.The upregulation of functional genes associated with nucleotide,energy,carbohydrate,amino acid metabolism,xenobiotic biodegradation,and metabolism:atrazine degradation was observed in biochar treatments.The biochar amendments significant

关 键 词：CHLORPYRIFOS ATRAZINE DISSIPATION Uptake kinetics BIOCHAR 

分 类 号：X59[环境科学与工程—环境工程]