抗阻运动激活Piezo1/AMPK/PGC-1α缓解小鼠废用性骨骼肌萎缩  

Resistance Exercise Activates Piezo1/AMPK/PGC-1α,Ameliorating Disuse-Induced Skeletal Muscle Atrophy in Mice

作　　者：韩东生[1] 梁擎宇[2] 石晓峰[1] HAN Dong-Sheng;LIANG Qing-Yu;SHI Xiao-Feng(College of Physical Education,Shanxi University,Taiyuan 030006,China;Equipment Department,The Second Hospital of Shanxi Medical University,Taiyuan 030001,China)

机构地区：[1]山西大学体育学院,太原030006 [2]山西医科大学第二医院设备处,太原030001

出　　处：《中国生物化学与分子生物学报》2025年第1期136-146,共11页Chinese Journal of Biochemistry and Molecular Biology

基　　金：山西省“1331工程”重点创新团队建设计划(No.1331KIRT)资助。

摘　　要：本研究旨在探究抗阻运动能否通过激活骨骼肌Piezo1/AMPK/PGC-1α信号通路,改善线粒体功能和促进肌生成,从而有效缓解废用性骨骼肌萎缩。采用8周龄雄性C57BL/6J小鼠,通过后肢石膏固定模拟废用性肌肉萎缩,并分为对照组、废用性肌萎缩组、废用性肌萎缩+抗阻运动组和废用性肌萎缩+抗阻运动+Piezo1抑制剂组。抗阻运动组进行为期8周的抗阻训练。通过Western印迹、免疫荧光染色、线粒体质量和功能检测等方法,评估骨骼肌质量、功能、线粒体状态和肌生成情况。抗阻运动使废用性肌萎缩小鼠的骨骼肌横截面积增加23%(P<0.01),骨骼肌相对质量增加11%(P<0.01)。抗阻运动使小鼠最长跑步距离平均增加206 m(P<0.01),最大承载力平均增加36.7g(P<0.01),平衡木爬行时间平均缩短1.5 s(P<0.01),同时上调了Piezo1、AMPK、PGC-1α的蛋白质表达水平(P<0.01)。此外,抗阻运动还增加了小鼠骨骼肌中MMP、TFAM、COXⅠ、CS、ATPB、ATPase、ATP、p-mTOR/mTOR、p-P70S6K/P70S6K、Pax7和MyoD的活性或蛋白质表达的水平(P<0.05,P<0.01)。而抑制Piezo1则降低了上述酶活性和蛋白质的表达水平(P<0.05,P<0.01)。抗阻运动通过激活Piezo1/AMPK/PGC-1α通路,提高了骨骼肌线粒体质量和功能,促进了蛋白质合成和肌生成,有效缓解了废用性骨骼肌萎缩。This study aims to investigate whether resistance exercise can effectively alleviate disuse skeletal muscle atrophy by activating the skeletal muscle Piezo1/AMPK/PGC-1αsignaling pathway,improving mitochondrial function and promoting myogenesis.8-week-old male C57BL/6J mice were used.Disuse muscle atrophy was simulated by hind limb plaster fixation,and the mice were divided into the control group,the disuse muscle atrophy group,the disuse muscle atrophy+resistance exercise group,and the disuse muscle atrophy+resistance exercise+Piezo1 inhibitor group.The resistance exercise group underwent an 8-week resistance training program.Methods such as Western Blotting,immunofluorescence staining,and mitochondrial mass and function detection were employed to evaluate skeletal muscle mass,function,mitochondrial status,and myogenesis.Resistance exercise significantly increased the cross-sectional area of skeletal muscle in mice with disuse muscle atrophy by 23%(P<0.01)and the relative mass of skeletal muscle by 11%(P<0.01).Resistance exercise led to an average increase of 206 m in the longest running distance of mice(P<0.01),an average increase of 36.7 g in the maximum bearing capacity(P<0.01),and an average reduction of 1.5 s in the balance beam crawling time(P<0.01).Meanwhile,it upregulated the expression of the protein levels of Piezo1,AMPK,and PGC-1α(P<0.01).In addition,resistance exercise increased the activity or protein expression levels of MMP,TFAM,COXⅠ,CS,ATPB,ATPase,ATP,p-mTOR/mTOR,p-P70S6K/P70S6K,Pax7,and MyoD in skeletal muscle(P<0.05,P<0.01).However,inhibiting Piezo1 decreased the expression of the above enzyme activities and protein levels(P<0.05,P<0.01).Resistance exercise alleviates disuse skeletal muscle atrophy effectively by activating the Piezo1/AMPK/PGC-1αpathway,improving skeletal muscle mitochondrial quality and function,and promoting protein synthesis and myogenesis.

关 键 词：抗阻运动 压电式机械敏感离子通道组件1 废用性骨骼肌萎缩 肌生成 

分 类 号：Q445[生物学—生理学]