聚多巴胺/MXene纳米复合材料对感染性创面的修复作用  

Effect of polydopamine/MXene nanocomposites on the repair of infectious wounds

作　　者：吴玲玲 何唯 王凯[2] 沐彩云 WU Lingling;HE Wei;WANG Kai;MU Caiyun(School of Acupuncture and Tuina,Anhui University of Chinese Medicine,Hefei 230012;The First Clinical College,Anhui University of Chinese Medicine,Hefei 230031)

机构地区：[1]安徽中医药大学针灸推拿学院,合肥230012 [2]安徽中医药大学第一临床学院,合肥230031

出　　处：《北京生物医学工程》2025年第1期39-48,共10页Beijing Biomedical Engineering

基　　金：安徽省自然科学基金(2108085QE204);安徽高校自然科学研究项目(KJ2020A0418);校高层次人才支持计划重点项目(2020rczd002)资助。

摘　　要：目的皮肤创面的形成不仅破坏皮肤的完整性,还大大增加细菌感染的风险,因此本文拟制备一种兼具光热效应、优良生物相容性及高效抗菌促愈合的纳米复合材料,以对感染性创面的治疗提供参考。方法首先将聚多巴胺和MXene复合,所得材料分别记为P 1M 2、P 1M 1和P 2M 1,并进行材料表征(透射电镜和X射线光电子能谱仪器)及性能检测(光热性能、细胞毒性和抗菌性能)。然后构建大鼠感染性创面模型,随机分为非处理组、莫匹罗星组、P 1M 2组(40、60、80和100μg/mL)和艾灸组。7 d后计算残留菌落数并观察创面愈合情况。结果成功制备PM纳米复合材料,其中P 1M 2、P 1M 1颗粒形态更均匀。相较于MXene,PDA修饰后的PM复合材料具有更优的光热性能。细胞实验显示,MXene经过PDA修饰后进一步提升了生物相容性。体外抗菌实验表明PM纳米复合材料具明显的抗菌性能,且P 1M 2组颗粒浓度越高,生物膜清除和破坏细菌膜通透性效果越好。动物实验中显示莫匹罗星、P 1M 2和艾灸组均能有效清除创面细菌并对感染性创面的修复作用明显。在第7天,P 1M 2-60和艾灸组促愈合效果优于莫匹罗星组,治疗温度相近的P1M2-60组(47.8℃)和艾灸组(47.5℃)促愈合疗效也接近。结论本研究制备了具有优良的光热性能和生物相容性及高效抗菌性能的PM纳米复合材料。动物实验结果不仅表明45~50℃的治疗温度更有利于感染性创面的愈合,也表明PM纳米复合材料具有实际应用潜力。该研究为感染性创面修复提供了一定的数据基础。Objective The formation of skin wounds not only destroys the integrity of the skin,but also greatly increases the risk of bacterial infection,so this study intends to prepare a nanocomposite material with photothermal effect,excellent biocompatibility and efficient antimicrobial healing,so as to provide a reference for the treatment of infectious wounds.Methods Firstly,polydopamine and MXene were composite,and the obtained materials were recorded as P 1M 2,P 1M 1 and P 2M 1,respectively.And the material characterization(transmission electron microscopy and X-ray photoelectron spectroscopy)and performance testing(photothermal performance,cytotoxicity and antibacterial properties)were carried out.Then the infectious wound model of rats was constructed and randomly divided into control group,mupirocin group,P 1M 2 group(40,60,80 and 100μg/mL)and moxibustion group.After 7 days,the number of residual colonies was calculated and the wound healing was observed.Results PM nanocomposites were successfully prepared,and the morphology of P 1M 2 and P 1M 1 particles was more uniform.Compared with MXene,PDA-modified PM composites have better photothermal properties.Cell experiments confirmed that MXene was more biocompatible after PDA modification.In vitro antimicrobial experiments showed that PM nanocomposites had obvious antibacterial properties,and the higher the particle concentration in the P 1M 2 group,the better the effect of biofilm removal and destruction of bacterial membrane permeability.Animal experiments showed that mupirocin,P 1M 2 and moxibustion groups could effectively remove wound bacteria and have a significant effect on the repair of infectious wounds.On the 7th day,the healing effect of the P 1M 2-60 and moxibustion groups was better than that of the mupirocin group,the P1M2-60 group(47.8℃)and moxibustion group(47.5℃)with similar treatment temperature also had similar healing efficacy.Conclusions In this study,PM nanocomposites with excellent photothermal properties,biocompatibility and high-efficien

关 键 词：聚多巴胺/MXene 光热治疗 生物相容性 创面愈合 艾灸 

分 类 号：R318.08[医药卫生—生物医学工程]