贡柑和沃柑E3泛素连接酶U-box基因的克隆  

Cloning of E3 Ubiquitin Ligase U-box Gene in Citrus reticulata cv.Gonggan and Orah mandarin

作　　者：吴少平 覃飞 廖金燕 冼睿芊 WU Shao-ping;QIN Fei;LIAO Jin-yan(School of Life Sciences,Zhaoqing University,Zhaoqing,Guangdong 526061;Fruit Tree Research Institute of Guangdong Academy of Agricultural Sciences,Guangzhou,Guangdong 510610)

机构地区：[1]肇庆学院生命科学学院,广东肇庆526061 [2]广东农业科学院果树研究所,广东广州510610

出　　处：《安徽农业科学》2025年第3期101-104,共4页Journal of Anhui Agricultural Sciences

基　　金：国家级大学生创新创业训练计划项目(202310580011);肇庆市科技创新指导类项目(2023040308015)。

摘　　要：为了研究贡柑和沃柑无籽形成的分子机理,选取了贡柑和沃柑作为研究对象,成功克隆了它们的E3泛素连接酶U-box基因。通过测序结果分析,发现贡柑和沃柑的U-box基因存在15个核苷酸差异,导致贡柑中有4个氨基酸发生了改变。通过序列比对,发现沃柑和无籽沙糖橘的E3泛素连接酶U-box基因相似度为98.19%,贡柑和无籽沙糖橘的E3泛素连接酶U-box基因相似度为97.72%,而沃柑和贡柑的E3泛素连接酶U-box基因相似度高达99.47%。In order to study the molecular mechanism of seedless formation in Citrus reticulata cv.Gonggan and Orah mandarin,Citrus reticulata cv.Gonggan and Orah mandarin were selected as research objects,and their E3 ubiquitin ligase U-box genes were successfully cloned.After analyzing the sequencing results,it was found that there were 15 nucleotide differences in the U-box genes of Citrus reticulata cv.Gonggan and Orah mandarin,resulting in changes in 4 amino acids in Citrus reticulata cv.Gonggan.Through sequence alignment,it was found that the similarity of E3 ubiquitin ligase U-box genes between Orah mandarin and Citrus reticulata‘Wuzishatangju’was 98.19%,while the similarity of E3 ubiquitin ligase U-box genes between Citrus reticulata cv.Gonggan and Citrus reticulata‘Wuzishatangju’was 97.72%.The similarity of E3 ubiquitin ligase U-box genes between Orah mandarin and Citrus reticulata cv.Gonggan was as high as 99.47%.

关 键 词：贡柑 沃柑 E3泛素连接酶U-box 

分 类 号：S666[农业科学—果树学]