白菜类蔬菜遗传转化研究进展  

Research progress on genetic transformation of Brassica rapa vegetables

作　　者：张家新 张文静 路翠玲 苏贺楠 赵艳艳[2] 魏小春[2] 杨双娟[2] 王志勇[2] 冯健起 张晓伟[2] 原玉香[2] 袁敬平 ZHANG Jiaxin;ZHANG Wenjing;LU Cuiling;SU Henan;ZHAO Yanyan;WEI Xiaochun;YANG Shuangjuan;WANG Zhiyong;FENG Jianqi;ZHANG Xiaowei;YUAN Yuxiang;YUAN Jingping(College of Horticulture and Landscape Architecture,Henan University of Science and Technology,Xinxiang 453003,Henan,China;Vegetable Research Institute,Henan Academy of Agricultural Sciences,Zhengzhou 450002,Henan,China;Zhengzhou Institute of Agriculture Science and Technology,Zhengzhou 450006,Henan,China;Kaifeng Academy of Agriculture and Forestry Sciences,Kaifeng 475004,Henan,China)

机构地区：[1]河南科技学院园艺园林学院,河南新乡453003 [2]河南省农业科学院蔬菜研究所,郑州450002 [3]郑州市农业科技研究院,郑州450006 [4]河南省开封市农林科学研究院,河南开封475004

出　　处：《中国瓜菜》2025年第2期1-6,共6页China Cucurbits And Vegetables

基　　金：中原科技创新领军人才(244200510041);省科技研发计划联合基金优势学科重点项目(232301420024);国家自然科学基金(32202485,32472730)。

摘　　要：遗传转化技术是研究白菜类蔬菜基因功能和开展遗传育种的重要手段。通过适宜的遗传转化方法,可以将目标基因导入生物基因组,实现对其生物学特性和经济性状的改良。然而,白菜类蔬菜的遗传转化面临着再生能力差、转化效率低等问题。通过查阅国内外白菜类蔬菜遗传转化相关文献,对影响白菜类蔬菜遗传转化效率的因素(基因型、再生体系、转化方法等)进行了分析和总结,探讨了CRISPR/Cas9在遗传转化中的应用,并对其再生与遗传转化的前景进行了展望。Genetic transformation technology is an important toll for exploring the gene functions of Brassica rapa vege-tables and conducting genetic breeding.Through appropriate genetic transformation methods,target genes can be intro-duced into the genome to improve their biological characteristics and economic traits.However,the genetic transforma-tion of Brassica rapa vegetables faces challenges such as poor regeneration ability and low transformation efficiency.This paper reviews the relevant literature on genetic transformation of Brassica rapa vegetables both domestically and in-ternationally,analyzes and summarizes the factors affecting transformation efficiency(such as genotype,regeneration sys-tem and transformation method,etc.),discusses the application of CRISPR/Cas9 in genetic transformation,and provides prospects for regeneration and genetic transformation in the future.

关 键 词：白菜类蔬菜 遗传转化 再生体系 转化方法 CRISPR/Cas9 

分 类 号：S634[农业科学—蔬菜学]