甜瓜果实细胞壁修饰相关基因的筛选和分析  

Screening and analysis of genes related to fruit cell wall modification of muskmelon fruit

作　　者：梁任繁[1] 黄皓[1] 文俊丽[1] 廖云云 高小凤 韦传义 张曼[1] 吴永升[1] 周国列 周生茂[1] LIANG Renfan;HUANG Hao;WEN Junli;LIAO Yunyun;GAO Xiaofeng;WEI Chuanyi;ZHANG Man;WU Yongsheng;ZHOU Guolie;ZHOU Shengmao(Guangxi Academy of Agricultural Sciences,Nanning 530007,Guangxi,China;Nanning Vegetable Research Institute,Nanning 530021,Guangxi,China;Yulin Academy of Agricultural Sciences,Yulin 537006,Guangxi,China)

机构地区：[1]广西农业科学院,南宁530007 [2]南宁市蔬菜研究所,南宁530021 [3]玉林市农业科学院,广西玉林537006

出　　处：《中国瓜菜》2025年第2期16-24,共9页China Cucurbits And Vegetables

基　　金：国家重点研发计划项目(2023YFD1200201);广西科技重大专项(桂科AA17204041);广西科技计划项目(桂科攻1222009-3F,桂科转14125004-28);国家现代农业产业技术体系广西创新团队项目(nycytxgxcxtd-1--07)。

摘　　要：甜瓜细胞壁对果实的脆度和口感具有重要影响。为了阐明细胞壁修饰相关基因对果实发育影响的分子机制,以厚皮甜瓜桂蜜12号为试材,应用转录组学、生物信息学筛选和分析雌花授粉后5 d和20 d的果实生长差异表达的基因。结果显示,果实两个发育时期差异表达基因有2950个,上调1141个,下调1809个。在富集Q值最显著的前20个条目中,GO富集指向细胞组分、生物过程等所涉及细胞壁数目最多(8个,占40%);KEGG富集后它们指向代谢途径、遗传信息途径、环境信息处理、细胞过程等途径。筛选出多聚半乳糖醛酸酶、扩张蛋白、木葡聚糖内糖基转移酶、葡萄糖醛酸转移酶、激素合成酶等基因,推测它们共同参与细胞壁修饰,影响甜瓜果实品质。The cell wall of muskmelon plays an important role in the fruit’s crispness and taste.To clarify the molecular mechanisms of the effect of cell wall modification related genes on fruit development,the muskmelon varity Guimi No.12 was used as the test material,and transcriptomics and bioinformatics were applied to screen and analyze the differential expression genes in the fruit at 5 and 20 days after pollination of female flowers.The results showed that there were 2950 differential expression genes during the two fruit development stages,with 1141 genes up-regulated and 1809 down-regulated.Among the top 20 enriched terms with the most significant Q-values,the GO enrichment pointed to cellular components and biological processes,with the highest number of cell wall related terms(8,accounting for 40%).KEGG enrichment indicated pathways related to metabolism,genetic information proocessing,environmental information processing,and cellular processes.Genes such as polygalactulose synthase,expansion protein,xyloglucan glycosyltransferase,glucurono-syltransferase,and hormone synthase were selected,suggesting that these genes collectively participate in cell wall mod-ification,affecting the quality of muskmelon fruit.

关 键 词：甜瓜 细胞壁修饰 差异表达基因 转录组学 生物信息学 

分 类 号：S652[农业科学—果树学]