冬瓜蜡质合成相关基因BhCER1的克隆与表达分析  

Cloning and expression analysis of BhCER1 gene involved in wax synthesis in wax gourd

作　　者：莫仁连 洪杰生 闫晋强 王宝琛 程晓欣 武涛 江彪[2] MO Renlian;HONG Jiesheng;YAN Jinqiang;WANG Baochen;CHENG Xiaoxin;WU Tao;JIANG Biao(College of Horticulture,Hunan Agricultural University,Changsha 410128,Hunan,China;Vegetable Research Institute,Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory for New Technology Research of Vegetable,Guangzhou 510640,Guangdong,China;South China Normal University,Guangzhou 510631,Guangdong,China)

机构地区：[1]湖南农业大学园艺学院,长沙410128 [2]广东省农业科学院蔬菜研究所·广东省蔬菜新技术研究重点实验室,广州510640 [3]华南师范大学,广州510631

出　　处：《中国瓜菜》2025年第2期32-39,共8页China Cucurbits And Vegetables

基　　金：广东省级乡村振兴战略专项资金种业振兴项目(2024-NPY-00-024);广东省科技计划项目(2023A0505090005)。

摘　　要：CER1基因在植物表皮蜡质的生长发育过程中发挥了重要的调控作用。为研究冬瓜CER1基因的生物学功能,利用同源序列克隆法克隆得到全长为1866 bp的BhCER1基因。该基因编码621个氨基酸,等电点为8.29,其蛋白质包含55个潜在磷酸化位点。蛋白质结构分析显示,该蛋白主要由无规则卷曲和α-螺旋构成。启动子序列分析表明,BhCER1启动子中包括与干旱、低温、盐胁迫等多种逆境响应相关的调控元件。进化分析表明,冬瓜BhCER1与同为葫芦科的甜瓜(CmCER1)和黄瓜(CsCER1)亲缘关系较近。qRT-PCR结果显示,BhCER1基因在冬瓜果皮中的表达量最高,其次是果肉、雌花、雄花、叶片、茎,而在根部基本无表达。综上所述,BhCER1基因可能在冬瓜果实蜡质合成过程中发挥重要作用,其具体的生物学功能尚待开展深入的系统研究。The CER1 gene plays an important role in the development of plant cuticular wax.In order to investigate the biological function of CER1 gene in wax gourd,the full-length of BhCER1 gene with a total length of 1866 bp was cloned using a homologous sequence cloning method.This gene encoded a protein of 621 amino acids with an isoelectric point of 8.29,and contained 55 potential phosphorylation sites.Protein structure analysis indicated the BhCER1 protein primarily consists of random coil andα-helix.Promoter sequence analysis showed that the promoter of BhCER1 contained regulatory elements associated responses to various abiotic stresses,such as drought,low temperature,and salt stress.Phylogenetic analysis showed that BhCER1 in wax gourd was closely related to CmCER1 in melon and CsCER1 in cucumber,both belonged to the Cucurbitaceae family.The results of qRT-PCR demonstrated that BhCER1 was most highly expressed in the fruit peel,followed by the fruit flesh,female flower,male flower,leaf,and stem,with negligible expression in the root.The above studies suggest that BhCER1 may play an important role in the fruit wax synthesis process of wax gourd.However,further systematic research is need to elucidate its specific biological functions.

关 键 词：冬瓜 果面蜡质 BhCER1 表达分析 

分 类 号：S642.3[农业科学—蔬菜学]