番茄叶面积的遗传分析和BSA-QTL定位  

Genetic analysis and BSA-QTL mapping of tomato leaf area

作　　者：李珊珊[1] 梁盼 孟繁艺 马梦秋 胡体旭 梁燕[1] 蔡义勇 战祥强 LI Shanshan;LIANG Pan;MENG Fanyi;MA Mengqiu;HU Tixu;LIANG Yan;CAI Yiyong;ZHAN Xiangqiang(College of Horticulture,Northwest A&F University,Yangling 712100,Shaanxi,China;Shaanxi Jinpeng Seedling Co.,Ltd.,Yangling 712100,Shaanxi,China)

机构地区：[1]西北农林科技大学园艺学院,陕西杨凌712100 [2]陕西金鹏种苗有限公司,陕西杨凌712100

出　　处：《中国瓜菜》2025年第2期40-49,共10页China Cucurbits And Vegetables

基　　金：宁夏回族自治区重点研发计划项目(2022BBF01001-02);陕西省重点研发计划项目(2021LLRH-07)。

摘　　要：叶面积是番茄的重要农艺性状之一,直接影响光合效率和干物质的积累,从而对植株的生长发育和生产力产生重大影响。研究番茄叶面积遗传规律,并通过BSA-seq进行基因定位,可以为研究番茄叶面积的遗传与分子机制奠定理论基础。以叶面积差异明显的21GA04(P_(1))和19GA13(P_(2))作为亲本,构建了P_(1)、P_(2)、F_(1)、F_(2)、BCP_(1)和BCP_(2)六世代群体。利用ImageJ软件测定番茄六世代群体叶面积。采用主基因与多基因混合遗传模型对番茄叶面积进行遗传分析;同时,通过集群分离分析法(BSA)检测极端材料及亲本基因型,并结合QTL-seq(SNP-index关联分析法)对叶面积进行关联基因的初步定位。在F_(2)、BCP_(1)和BCP_(2)3个分离群体中,番茄叶面积均呈现偏正态分布。番茄叶面积的最适遗传模型为MX2-ADI-AD,表明该性状受2对加性-显性-上位性主基因和加性-显性多基因控制。采用SNP-index关联分析法共检测到3个QTL区间,分别位于番茄1号染色体的89.0~89.8 Mb、8号染色体的2.1~4.5 Mb和9号染色体的63.9~64.8 Mb。结果表明,番茄的叶面积受主基因和多基因的共同调控,且与叶面积性状相关的区间位于1号、8号和9号染色体上,推测其中SlSAUR20和SlSAUR21基因为关联叶面积性状的候选基因。Leaf area is one of the important agronomic traits in tomato that directly influences photosynthetic efficiency and material accumulation,thus significantly affecting plant growth and productivity.Studying the inheritance patterns of tomato leaf area and performing gene mapping through BSA-seq can provide a theoretical foundation for understanding the genetic and molecular mechanisms of leaf area in tomato.In this study,two parental lines with significant differences in leaf area,21GA04(P1)and 19GA13(P_(2)),were selected to construct a population comprising six generations:P_(1)、P_(2)、F_(1)、F_(2)、BCP_(1)and BCP_(2).ImageJ software was used to measure the leaf area across these six generations.Genetic analysis of tomato leaf area was performed using a mixed genetic model combining major genes and polygenes.Additionally,bulked segregation analysis(BSA)was employed to assess the genotypes of extreme materials and parents,while QTL-seq(SNP-index association analysis)was used for preliminary localization of leaf area associated genes.The leaf area in the F_(2),BCP1 and BCP_(2) populations showed a skewed normal distribution.The most suitable genetic model for tomato leaf area is MX2-ADI-AD,indicating that this trait is controlled by two pairs of additive-dominant epistatic major genes and additive-dominant polygenes.SNP-index association analysis identified three QTL intervals,located on chromosome 1(89.0-89.8 Mb),chromosome 8(2.1-4.5 Mb),and chromosome 9(63.9-64.8 Mb).These results suggest that tomato leaf area is governed by both major genes and polygenes,with three associated regions located on chromosomes 1,8 and 9,and SlSAUR20 and SlSAUR21 are proposed as candidate genes associated with leaf area trait.

关 键 词：番茄 叶面积 遗传分析 QTL定位 

分 类 号：S641.2[农业科学—蔬菜学]