可栽培黄色羊肚菌品种的鉴定与系统发育分析  

Identification and phylogenetic analysis of cultivable yellow morel mushrooms

作　　者：张文昌 孙文化 王海彦 尹琪 孔维丽[2] 何培新 刘伟 ZHANG Wenchang;SUNWenhua;WANG Haiyan;YIN Qi;KONG Weili;HE Peixin;LIU Wei(College of Food and Biological Engineering,Zhengzhou University of Light Industry,Zhengzhou 450001,Henan,China;Institute of Mushroom,Henan Academy of Agricultural Sciences,Zhengzhou 450002,Henan,China;Luoyang Wilderness Ecological Agriculture Co.,Ltd.,Luoyang 471000,Henan,China;Kunming Institute of Botany,Chinese Academy of Sciences/Yunnan Key Laboratory for Fungal Diversity and Green Development,Kunming 650201,Yunnan,China)

机构地区：[1]郑州轻工业大学食品与生物工程学院,郑州450001 [2]河南省农业科学院食用菌研究所,郑州450002 [3]洛阳大荒野生态农业有限公司,河南洛阳471000 [4]中国科学院昆明植物研究所云南省真菌多样性与绿色发展重点实验室,昆明650201

出　　处：《中国瓜菜》2025年第2期59-65,共7页China Cucurbits And Vegetables

基　　金：河南省现代农业产业技术体系(HARS-22-08-S,HARS-22-08-G1)。

摘　　要：采集栽培驯化基地的黄色羊肚菌子囊果样品,经组织分离获得了菌丝体培养物,进而采用常规的形态学特征结合多基因系统发育分析,包括内转录间隔区(internal transcribed spacers,ITS)、延长因子1α(elongation factor1-alpha,EF1-α)、RNA聚合酶II大小亚基(RNA polymerase II subunit 1/2,RPB1/RPB2)4个DNA片段,对3个可栽培黄色羊肚菌品种进行分类鉴定。子囊果的宏观和微观形态特征表明,驯化栽培羊肚菌属于黄色羊肚菌类群物种;基于4个DNA片段的系统发育分析表明,3个驯化羊肚菌品种序列与10个黄色羊肚菌参考序列一起被聚类在黄色羊肚菌支系(Esculenta clade)内,与Mes-21标本被聚类在一个单系发育类群(支持率100%),表明其属于Mes-21系统发育学种。这是首次被确认的黄色羊肚菌人工栽培种类,将有效地推动我国黄色羊肚菌人工栽培技术发展和规模化栽培应用。The ascocarps of yellow morel samples from a cultivation and domestication base were collected,and mycelial isolates were obtained through tissue separation.Conventional morphological characters combined with the analysis of concatenated sequences of internal transcribed spacers(ITS),elongation factor 1-alpha(EF1-α),RNA polymerase II subunit 1/2(RPB1/RPB2)were used to classify and identify three cultivated yellow morel varieties.The macro-and micro-morphological characteristics of the ascocarps supported their identities of yellow morels.Phylogenetic analysis based on four gene fragments showed that the sequences of the three domesticated morel varieties clustered together with 10 morel reference sequences in the Esculenta clade,and with the Mes-21 in a monophyletic group(support rate 100%),indicating that they belong to the Mes-21 phylogenetic species..This is the first confirmed artificial cultivated species of Esculenta clade,which will effectively promote the development of yellow morel artificial cultivation technology and its large-scale cultivation applications in China.

关 键 词：羊肚菌 支系 驯化栽培 系统发育学种 Mes-21 

分 类 号：S646.7[农业科学—蔬菜学]