海南石斛自交结实率及无菌播种的研究  

Study on Self-fertilization Rate and Aseptic Sowing of Dendrobium hainanense

作　　者：符洁 季玉池 冯加钦 梁昕景 王祥锐 李香 邢艳冰 Fu Jie;Ji Yuchi;Feng Jiaqin;Liang Xinjing;Wang Xiangrui;Li Xiang;XingYanbing(Sanya Academy of Forestry,Sanya Hainan572000)

机构地区：[1]三亚市林业科学研究院,海南三亚572000

出　　处：《热带林业》2024年第4期29-31,37,共4页Tropical Forestry

基　　金：海南省自然科学基金面上项目(No.322MS157)“海南濒危兰花无性繁殖及野外回归”。

摘　　要：文章以海南石斛野生种进行人工自交授粉获得果荚,通过授粉时期和授粉方式处理比较,探讨了人工授粉对海南石斛结实率和无菌消毒及播种培养基萌发率的影响,对所获得的海南石斛种子经过消毒后无菌条件下进行播种繁育。结果表明:(1)不同自交方式对海南石斛结实率存在差异,结实率范围在0~76.67%;自交结实率与亲本开花时间有关,和自交时间无关。(2)果荚的消毒无需清水冲洗,用浓度为0.1%的HgCl_(2)(20min)的效果最好,污染率最低。(3)无菌播种培养基为1/2MS+6-BA0.5mg/L+NAA0.2mg/L+10%香蕉+0.5%碳+10%白糖时,萌发率较高。In this paper,the effects of artificial pollination on seed setting rate,sterile disinfection,and germination rate of seeding medium were discussed by comparing treatments of pollination periods and pollination methods of artificially self-pollinated Dendrobium hainanense wild species,and the seeds obtained were sown and propagated under aseptic conditions after disinfection.The results showed that:(1)There were differences in the seed setting rate of D.Hainanense between different self-inbred methods while the number ranged from 0%to 76.67%.The self-fertilization rate was related to the flowering time of the parents but irrelevant to the self-inbred time.(2)It was unnecessary for disinfection to rinse the pods with water,and the least polluted way was a 20-minute sanitization of 0.1%HgCl_(2).(3)The aseptic seeding medium with a higher germination rate was:1/2MS+6-BA0.5mg/L+NAA0.2mg/L+10%banana+0.5%carbon+10%sugar.

关 键 词：海南石斛 人工授粉 结实率 无菌播种 

分 类 号：S334.25[农业科学—作物遗传育种]