三叶草锈病发生的生理特性研究  

Study on the Physiological Characteristics of Trifolium pratense

作　　者：董帅 李琼芬 岳宁波 周敏 韩继镕 蒋兰兰 Dong Shuai;Li Qiongfen;Yue Ningbo;Zhou Min;Han Jirong;Jiang Lanlan(Qiannan Prefectural Agricultural Sciences Research Institute,Qiannan Guizhou 558000;Guizhou Vocational College of Agriculture,Guiyang Guizhou 551400)

机构地区：[1]贵州省黔南州农业科学研究院,贵州黔南558000 [2]贵州农业职业学院,贵州贵阳551400

出　　处：《热带林业》2024年第4期38-43,共6页Tropical Forestry

基　　金：黔科合服企(〔2024〕012)“黔南州科研基地种质资源圃建设项目”。

摘　　要：研究以感病和未感病的三叶草叶片为实验材料,利用考马斯亮蓝法和紫外吸收法测定2种三叶草叶片的可溶性蛋白含量,同时测定了2种叶片的丙二醛含量。结果表明,2种可溶性蛋白测定的实验方法所测结果基本一致,可溶性蛋白含量均是未感病叶片高于感病叶片,未感病叶片可溶性蛋白含量为39.98mg/g。感病叶片丙二醛含量低于未感病叶片,说明未感病叶片对锈病的耐受性及抗病性均高于感病叶片。因此,研究从生理特性上为三叶草抗病资源的利用和抗病品种培育奠定了基础。In this study,susceptible and non-susceptible Trifolium pratense leaves were used as experimental materials.The soluble protein contents of the two kinds of leaves were determined by the Coomassie blue staining and the ultraviolet absorption spectrophotometry,and the malondialdehyde(MDA)contents were also determined.It was shown that the results of the two soluble protein determination experimental methods were basically consistent.The soluble protein content of non-susceptible leaves(39.98 mg/g)was higher than that of susceptible leaves.The MDA content of susceptible leaves was lower than that of non-susceptible leaves,indicating that the non-susceptible leaves were more tolerant and resistant to rust.Therefore,this study lays a physiological foundation for the utilization of disease-resistant resources and the cultivation of new disease-resistant varieties.

关 键 词：三叶草 锈病 可溶性蛋白 丙二醛 

分 类 号：S541.2[农业科学—作物学]