线粒体Cyt b基因对九种穿山甲鉴别力的分析  

An Evaluation of Resolution of Mitochondrial Cyt b Gene for Identification of Nine Pangolin Species

作　　者：韩松霖 马跃[1,2] 李波 刘微[1,2] 穆佳童 徐艳春[1,2,3] 杨淑慧 HAN Songlin;MA Yue;LI Bo;LIU Wei;MU Jiatong;XU Yanchun;YANG Shuhui(College of Wildlife and Protected Area,Northeast Forestry University,Harbin,150040,China;National Forestry and Grassland Administration Detecting Center of Wildlife,Harbin,150040,China;National Forestry and Grassland Administration Research Center of Engineering and Technology for Wildlife Conservation and Utilization,Harbin,150040,China)

机构地区：[1]东北林业大学野生动物与自然保护地学院,哈尔滨150040 [2]国家林业和草原局野生动植物检测中心,哈尔滨150040 [3]国家林业和草原局野生动物保护与利用工程技术研究中心,哈尔滨150040

出　　处：《野生动物学报》2025年第1期50-62,共13页CHINESE JOURNAL OF WILDLIFE

基　　金：中央高校基本科研业务费专项资金项目(2572020DR10);国家自然科学基金项目(32170517)。

摘　　要：为优化穿山甲的分子鉴定方法,采用9种穿山甲54条Cyt b基因完整序列,评估了MEGA11软件中的7种模型和23种碱基替换类型所估算的遗传距离的物种分辨率、Cyt b基因全序列及各区域鉴别力。结果表明:K2P模型和p-distance模型基于颠换(T_(V))计算的遗传距离具有最优的物种分辨率;Cyt b基因全序列有较好的物种鉴别能力,其中,第301~400号和第851~950号的2个碱基序位片段组合时具有最高的物种分辨率,是鉴别9种穿山甲的最佳理论区段。现有的方法中推荐使用Kocher等的引物。今后在研发中采用多重PCR结合高通量测序对多片段组合进行分析,将会提高穿山甲的鉴别力。In order to optimize the molecular identification of pangolins,we evaluated species resolution of genetic distance estimated using seven models and 23 nucleotide substitution modes in MEGA 11 and the power of species identification of the whole and segments of Cyt b gene using 54 complete Cyt b sequences of nine pangolin species.The results showed that the intra-and inter-specific distance computed using K2P and p-distance model with transversion(TV)as substitution mode posed the greatest species resolution.The whole sequence of Cyt b gene had acceptable species identification power,but segments ranging combination between the 301^(st)~400^(th) and 851^(st)~950^(th )nucleotide showed optimal species identification power,is the best section for identifying nine species of pangolins.Among the existing methods,it is recommended to use the primers of Kocher et al.The above results suggest that use of multiple optimal segments would substantially improve the identification accuracy of pangolins given multiplex PCR and massive parallel sequencing approach are used.

关 键 词：穿山甲 细胞色素b基因(Cyt b) 遗传距离 物种鉴定 

分 类 号：Q953[生物学—动物学]