贵州兴义美花石斛基因组特征研究  

Genomic Characterization of Dendrobium loddigesii from Xingyi,Guizhou

作　　者：王维泽 周舒婷 杜致辉 晋宇轩 张朝君[1] 陈之林[1] WANG Weize;ZHOU Shuting;DU Zhihui;JIN Yuxuan;ZHANG Chaojun;CHEN Zhilin(Guizhou Horticultural Institute,Guizhou Academy of Agricultural Sciences/Guizhou Research Center of Horticultural Engineering Technology,Guiyang 550006,China;Natural Products Research Center of Guizhou Province,Guiyang 550014,China)

机构地区：[1]贵州省农业科学院园艺研究所/贵州省园艺工程技术研究中心,贵阳550006 [2]贵州省天然产物研究中心,贵阳550014

出　　处：《种子》2025年第1期66-71,共6页Seed

基　　金：贵州省基础研究(自然科学)项目(黔科合基础-ZK[2023]一般189);贵州省科技计划项目(黔科合服企[2022]005);贵州省农业科学院2024年省财政科研专项(种质资源专项)(黔农科种质资源[2024]);贵州省农业科学院青年基金项目(黔农科院青年基金[2022]30号)。

摘　　要：本研究以贵州兴义浓香型美花石斛(Dendrobium loddigesii)为材料,通过染色体制片、流式细胞术和二代高通量技术相互验证,分析贵州兴义浓香型美花石斛群体染色体数量、大小、倍性等特征。结果表明,染色体制片观察到贵州兴义美花石斛染色体数量为2 n=2 x=38,流式细胞术估算贵州兴义美花石斛基因组大小为1.79 Gb,根据高通量测序估算贵州美花石斛的基因组大小为1.70 Gb,重复序列比例为62.99%,杂合度为0.65%,GC含量为33.41%,贵州兴义美花石斛属于微杂合高重复基因组,是目前已知二倍体石斛中基因组最大的石斛,可作为探究石斛属物种演化及芳香型石斛育种研究的重要参考物种。In this study,Dendrobium loddigesii from Xingyi,Guizhou was used as the material to analyze the chromosome number,size and ploidy characteristics of the population of Dendrobium loddigesii from Xingyi,Guizhou by mutual verification of chromosome production,flow cytometry and second-generation high-throughput techniques.The results showed that the number of chromosomes of Dendrobium loddigesii from Xingyi,Guizhou was 2 n=2 x=38,the genome size of Dendrobium loddigesii from Xingyi,Guizhou was estimated to be 1.79 Gb by flow cytometry,the genome size of Dendrobium loddigesii from Xingyi,Guizhou was estimated to be 1.70 Gb by high-throughput sequencing,the proportion of repeats was 62.99%,the heterozygote was 0.65%,and the GC content was 33.41%,and the genome of the Dendrobium loddigesii from Xingyi,Guizhou was the largest diploid dendrobium with a microheterozygous high repeat genome,which could be used as an important reference species to explore the evolution of Dendrobium species and the breeding of aromatic Dendrobium loddigesii.

关 键 词：美花石斛 基因组 染色体制片 流式细胞术 二代高通量测序 

分 类 号：S567.239[农业科学—中草药栽培]