水稻种质苗期耐旱性评价及相关酶活性研究  

Evaluation of Drought Tolerance and Correlation of Enzyme Activity inRice Germplasm at Seedling Stage

作　　者：蒋京桂 黄世炎 黄成志 刘军化 雷树凡 吕直文 刘忠贤 刘鹏 蔡钟亚 JIANG Jinggui;HUANG Shiyan;HUANG Chengzhi;LIU Junhua;LEI Shufan;L Zhiwen;LIU Zhongxian;LIU Peng;CAI Zhongya(Chongqing Three Gorges University,Wanzhou Chongqing 404020,China;Chongqing Three Gorges Academy of Agricultural Sciences,Wanzhou Chongqing 404150,China)

机构地区：[1]重庆三峡学院,重庆万州404020 [2]重庆三峡农业科学院,重庆万州404150

出　　处：《种子》2025年第1期79-88,共10页Seed

基　　金：国家现代农业产业技术体系建设专项项目(CARS-01-77);重庆市现代农业产业技术体系(CQMAITS202301);重庆三峡农业科学院自立科技项目(Sxnky2023003)。

摘　　要：为探究水稻苗期耐性及相关酶活性情况,以31份水稻种质为试验材料,对其苗期干旱胁迫处理和常规处理下水稻株高、株总长、根长、茎叶鲜重、根鲜重、根数、茎叶干重和根干重等8个形态指标进行测定,同时分别测定超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性和丙二醛(MDA)含量,分析其与耐旱水平的相关性。结果表明,筛选出强耐旱性水稻种质材料5个(万恢45、303B、郑旱10号、万恢56、H6),中耐旱性水稻种质材料11个,弱耐旱性种质材料13个、干旱敏感型种质材料2个。比对31份水稻种质干旱胁迫处理和常规处理酶活性,SOD活性26个种质增高,5个降低;POD活性22个种质增高,9个降低;CAT活性28个种质增高,3个降低。而MDA含量呈相反趋势,7个种质值增高,24个降低。通过对水稻的耐旱水平和各酶活性相对值做相关性分析,发现耐旱水平与POD活性呈正相关,与MDA含量呈负相关。In order to investigate rice(Oryza sativa L.)seedling tolerance and related enzyme activities,8 morphological traits including plant height,plant total length,root length,stem and leaf fresh weight,root fresh weight,root number,stem and leaf dry weight and root dry weight were measured and analyzed in 31 rice germplasm materials under drought stress and conventional treatment.At the same time,the activities of antioxidant enzymes SOD,POD,CAT and MDA were measured respectively,and the correlation between them and drought tolerance level was analyzed.The results showed that 5 rice germplasm materials(Wanhui45,303B,Zhenghan No.10,Wanhui56,H6)were selected for strong drought tolerance,11 varieties for medium drought tolerance,13 varieties for weak drought tolerance and 2 varieties for drought sensitivity.Compared with 31 rice germplasms treated with drought stress and conventional treatment,SOD activity increased in 26 rice germplasms and decreased in 5 rice germplasms.POD activity increased in 22 germplasms,and 9 germplasms decreased;CAT activity increased in 28 germplasms and decreased in 3.MDA values showed the opposite trend,increasing in 7 germplasms and decreasing in 24 germplasms.Through the correlation analysis of drought tolerance level and the relative value of each enzyme activity,it was found that drought tolerance level was positively correlated with POD activity and negatively correlated with MDA value.

关 键 词：水稻 耐旱 形态指标 酶活性 

分 类 号：S511[农业科学—作物学]